Table 1.
Summary of partial purification of crude protease from B. subtilis isolate.
| Steps | Volume (ml) | Protein (mg/ml) | Total protein (mg) | Activity (U/ml) | Total activity (U) | Specific activity (U/mg) | Purification fold | Yield (%) |
|---|---|---|---|---|---|---|---|---|
| Crude | 150 | 0.777 | 116.55 | 141.8 | 21270 | 182.5 | 1 | 100 |
| 30% (NH4)2SO4 ppt. | 2 | 1.614 | 3.228 | 722.5 | 1445 | 447.64 | 2.45 | 6.8 |
| 60% (NH4)2SO4 ppt. | 2 | 2.284 | 4.568 | 5362.5 | 10725 | 2347.85 | 12.86 | 50.42 |
| 90% (NH4)2SO4 ppt. | 2 | 1.812 | 3.624 | 252.5 | 505 | 278.7 | 1.52 | 2.37 |
| DEAE- Cellulose | 1 | 0.249 | 0.249 | 2075.5 | 2075.5 | 8335.34 | 45.67 | 9.75 |