Cytotoxic Anomoian B and Aplyzanzine B, New Bromotyrosine Alkaloids from Indonesian Sponges

Abstract

Two new bromotyrosine derivatives, anomoian B (1) and aplyzanzine B (2), were isolated, respectively, from the organic extracts of a Verongida sponge belonging to the Hexadella genus and from a two-sponge association (Jaspis sp. and Bubaris sp.), both collected off the coast of Indonesia. The planar structure of 1 and 2 was determined by 1D and 2D NMR experiments and by high-resolution mass spectrometry, while their absolute stereochemistry was assigned by comparison with optical rotation values of known bromotyrosines and by chemical degradation. Both compounds showed moderate antiproliferative activity against a panel of different cancer cell lines. Their cytotoxic activity is facilitated through the induction of apoptosis, which is mediated neither by the generation of reactive oxygen species nor by the inhibition of histone deacetylases in these cell lines.

1. Introduction

Marine sponges belonging to the order Verongida are devoid of spicules, and their characterization is a real challenge in terms of taxonomy.¹ However, the bromotyrosine derivatives that are distinctive of this type of sponges² have been used as chemotaxonomic markers and have been proved to be a useful tool to facilitate taxonomic identification.³ Since the first report of (+)-aeroplysinin-1,³ marine sponges of the order Verongida have been found to produce many bromotyrosine derivatives such as aerothionin,⁴ purealidins,^5,6 purpurealidins,⁷ purpuramines,^6,8 aplysamines,³ pseudoceralidinone A,⁹ and purealines.¹⁰ Despite this, Verongida is not the only order of sponges where bromotyrosine derivatives have been found. Two-sponge associations, where at least one of the members belongs to the genus Jaspis, are also known to contain this kind of metabolites.¹¹

From an ecological point of view, this type of compounds has evolved as a defense system against natural predators, with the exception of some nudibranchs and mollusks that can feed on these sponges.¹²

Some of these alkaloids have been described as antiproliferative, apolipoprotein E modulators or cytotoxic and antibacterial agents.² Previous studies of their molecular mechanism of action revealed that psammaplin^13,14 is an inhibitor of histone deacetylases (HDACs), whereas the small bromotyrosine derivatives¹⁵ are potent apoptotic effectors.

2. Results and Discussion

In the course of our ongoing drug discovery program to find new antitumor agents from marine sponges,^16,17 extracts of two Indonesian samples were fractionated, guided by their cytotoxic activities, leading to the isolation of two new bromotyrosine derivatives. The first sample, an unknown species of the genus Hexadella (order Verongida) collected at Para Island in Indonesia, was extracted, and the soluble materials were subjected to reversed-phase chromatography to yield anomoian B (1, 0.037%, wet wt) as a colorless, amorphous solid together with the known bromotyrosine alkaloid aplysamine 4 (3) (Chart 1).¹⁴ Previous chemical studies of marine sponges of the genus Hexadella reported the isolation of bromotyrosine derivatives related to aerothionin and bisindole alkaloids.¹⁹⁻²¹

Chart 1.

The second sample, a two-sponge association of Jaspis sp. and Bubaris sp. collected in Pulau Saujung in Indonesia, was extracted to give an organic extract, which after bioguided fractionation yielded aplyzanzine B (2, 0.057%, wet wt) as a brown amorphous solid. Although there are earlier reports of chemical studies of Jaspis genus sponges associated with another sponge genus,¹¹ this work constitutes the first study of a two-sponge association of Jaspis sp. and Bubaris sp. As far as we know, there is a just one chemical study of a sponge belonging to Bubaris genus that describes the isolation of a sesquiterpenoid isocyanide.^22,23

The (+)-low-resolution electrospray ionization mass spectra (LRESIMS) data of 1 [[α]_D + 10.5° (c 1.0, CH₃OH)] showed the [M + H]⁺ ion as a cluster at m/z 740, 742, 744, 746, and 748 in a 1:4:6:4:1 ratio, indicating the presence of four bromine atoms in the molecule. This was verified by subsequent (+)-high-resolution electrospray ionization time-of-flight mass spectrometry (HRESITOFMS) of the first isotopic peak at m/z 739.9355 [M + H]⁺ (calcd for C₂₅H₃₄⁷⁹Br₄N₃O₃m/z 739.9328), which in combination with its NMR data (Table 1) allowed us to propose the molecular formula of 1 as C₂₅H₃₃Br₄N₃O₃.

Table 1. NMR Data of Anomoian B (1) in CD₃OD and Aplyzanzine B (2) in CDCl₃.

1			2
pos.	δC type	δHm (J in Hz)	pos.	δC type	δHm (J in Hz)
1	134.5 C		1	133.4 C
2	135.2 CH	7.51s	2	133.6 CH	7.40s
3	119.3 C		3	118.5 C
4	155.1 C		4	153.9 C
5	119.3 C		5	118.5 C
6	135.2 CH	7.51s	6	133.6 CH	7.40s
7	34.3 CH2	3.36m	7	34.1 CH2	3.22dd (12.4, 3.6)
		3.00m			3.09dd (12.4, 11.3)
8	70.5 CH	3.91dd (11.2, 4.6)	8	60.4 CH	4.97dd (11.3, 3.6)
9	167.2 C		9	166.8 C
10	38.0 CH2	3.51m	10	54.9 CH2	5.06d (17.5)
		3.28m			4.19d (17.5)
11	30.6 CH2	1.84m	11	189.3 C
		1.70m	12	132.5 C
12	71.6 CH2	3.87m	13	132.7 CH	8.03s
		3.75m	14	118.9 C
13	153.4 C		15	157.0 C
14	119.4 C		16	118.9 C
15	134.4 CH	7.57s	17	132.7 CH	8.03s
16	135.1 C		18	69.9 CH2	4.13t (5.5)
17	134.4 CH	7.57s	19	25.3 CH2	2.32dq (11.2, 5.5)
18	119.4 C		20	55.5 CH2	3.42m
19	30.3 CH2	2.99m	21	40.0 CH3	2.97s
20	59.1 CH2	3.33m	22	40.0 CH3	2.97s
21	42.5 CH3	2.99s	23	42.9 CH3	2.88s
22	42.5 CH3	2.99s	24	42.9 CH3	2.88s
23	43.6 CH3	2.93s	25	60.7 CH3	3.85s
24	43.6 CH3	2.93s	26	37.6 CH3	2.75s
25	61.1 CH3	3.77s

Maximum UV absorptions at 276 and 284 nm indicated a possible substituted benzoic system, whereas the diagnostic IR vibration at 1673 cm^–1 suggested the presence of an amide carbonyl group. The ¹³C NMR spectrum (see Table 1) disclosed signals owing to nine sp² quaternary carbons including an amide (δ_C 167.2), four sp² methines, one sp³ methine, six sp³ methylenes, and five methyls.

Analysis of the ¹H–¹H correlation spectroscopy (COSY) along with the Distortionless Enhancement by Polarization Transfer (DEPT)-135-edited heteronuclear single-quantum correlation (HSQC) spectra of 1 revealed the presence of three spin systems: (a) 2-aminoethyl (δ_C7 34.3/δ_H7 3.36/3.00 and δ_C8 70.5/δ_H8 3.91), (b) 3-aminopropanol (δ_C10 38.0/δ_H10 3.51/3.28, δ_C11 30.6/δ_H11 1.84/1.70, and δ_C12 71.6/δ_H12 3.87/3.75), and (c) 2-aminoethyl (δ_C19 30.3/δ_H22 2.99 and δ_C20 59.1/δ_H23 3.33) (Figure 1A). Additionally, singlets observed in the ¹H NMR spectrum of 1 were assigned to a methoxy group (δ_OMe 61.1/δ_OMe 3.77), two dimethylamino groups (δ_C21,22 42.5/δ_H21,22 2.99 and δ_C23,24 43.6/δ_H23,24 2.93), and four aromatic protons (δ_C2,6 135.2/δ_H2,6 7.51 and δ_C15,17 134.4/δ_H15,17 7.57, 2H each), characteristic of two symmetrically dibromine-substituted aromatic rings. The three spin systems and the other groups were connected by the aid of a heteronuclear multiple-bond correlation (HMBC) experiment (see Figure 1A): (a) The methoxy group was located at C4 of one of the dibromine aromatic rings by the HMBC correlation between the Me25 protons at δ_H 3.77 and the quaternary aromatic carbon C4 at δ_C 155.1. Furthermore, the HMBC correlations between the aromatic protons H-2/6 and the C4 and C7 carbons connected the aromatic ring to the spin system a; correlations between the dimethylamino protons H21/H22 at δ_H 2.99 and C8 at δ_C8 70.5 and between the proton H8 at δ_H8 3.91 and the carbonyl amide C9 allowed us to identify an N,N′-dimethylamino 4-methoxy-3,5-dibrominetyrosine unit, or “tyrosine” fragment, in 1. (b) 3-Aminopropanol (spin b) was linked on one side to the former tyrosine fragment by an HMBC correlation between the methylene H₂10 protons at δ_H 3.51/3.28 and the carbonyl amide C9 and on the other side to the second dibromine-substituted aromatic ring by the key long-range correlation between the methylene H₂12 at δ_H 3.87/3.75 and the aromatic oxygenated carbon C13 at δ_C 153.4. (c) Finally, the second 2-aminoethyl group (spin c) was connected on one side to the second dibromine-substituted aromatic ring by HMBC correlations between the aromatic protons H15/H17 at δ_H 7.57 and the methylene carbon C19 (δ_C 30.3) and on the other side to the dimethylamino moiety through the HMBC correlation between methyl protons H23/24 at δ_H 2.93 and the methylene carbon C20. These correlations allowed us to identify an N,N′-dimethylamino-3,5-dibrominetyramine unit (the “tyramine” fragment).

Figure 1.

A) Selected two-dimensional (2D) NMR correlations of compound 1. (B) (+)-HRESIMS of the fragments detected in a MS/MS experiment of 1.

Nuclear Overhauser effect (NOE) correlations between protons H2/6 and H7/8, H21/22 and H8, and H15/17 and H19/20 observed in a rotating-frame Overhauser effect spectroscopy (ROESY) experiment of 1 were consistent with the proposed structure (Figure 1A). Moreover, the proposed planar structure of 1 was further confirmed by the presence of diagnostically important peaks at m/z 333.9432 and 319.9276 detected in the (+)-HRESIMS/MS experiment of 1 (Figure 1B), corresponding to selective fragmentation arising from the loss of both the tyrosine and tyramine fragments. All of this information allowed us to establish the structure of 1, named anomoian B, as having a framework (tyrosine)–(“3-aminopropanol”)–(tyramine), similar to that found in the purpuramines.^6,8

A similar approximation was chosen for the structure elucidation of 2, namely, as aplyzanzine B. The molecular formula of aplyzanzine B (2) was determined as C₂₆H₃₄Br₄N₃O₄ based on the [M + H]⁺ ion peak observed at m/z 767.9285 in its (+)-HRESITOF mass spectrum and on the NMR data (Table 1).

The ¹H and ¹³C NMR spectral data (Table 1) of 2 showed the presence of the same N,N′-dimethylamino-4-methoxy-3,5-dibrominetyrosine unit (spin a), a 3-aminopropanol entity (δ_C18 69.9/δ_H18 4.13, δ_C19 25.3/δ_H19 2.32, and δ_C20 55.5/δ_H20 3.42) (spin c), a second dimethylamino group, and another symmetrically dibromine-substituted aromatic ring as in 1. Furthermore, the NMR spectra of 2 showed the presence of additional diastereotopic methylene protons (δ_C10 54.9/δ_H10 5.06 and 4.19) (spin b), an N-methylamide group (δ_C26 37.6/δ_H26 2.75), and a ketone carbonyl group (δ_C 189.3) instead of the second 2-aminoethyl group present in 1.

Key HMBC correlations allowed us to identify the presence of a −N(Me)–CH₂–CO– unit linked to the N,N′-dimethylamino-4-methoxy-3,5-dibrominetyrosine unit (tyrosine fragment) as follows:

• (1)HMBC cross peaks from the methylamide protons at δ_H26 2.75 to the carbonyl amide carbon C9 (δ_C 166.8) and the methylene carbon C10 (δ_C 54.9) and
• (2)HMBC correlations from the diastereotopic methylene protons H₂10 (δ_H 5.06 and 4.19) (spin b) to the carbon C9 and the ketone carbonyl carbon C11 (δ_C 189.3).

Moreover, long-range correlations from the aromatic protons H13/17 to the ketone carbonyl carbon C11 allowed us to link the −N(Me)–CH₂–CO– unit to the second dibromine aromatic ring on the other side. Thus, an N-methylamino-3,5-dibrominetyramine unit (tyramine fragment) was identified as a substructure of 2.

The second N,N′-dimethylamino group was linked to the 3-aminopropanol unit (spin c) by the HMBC correlations from methyl protons H23/24 at δ_H 2.88 to the methylene carbon C20 (δ_C 55.5). Finally, spin c was in turn connected to the second dibromine aromatic ring by the long-range correlations from the methylene protons H₂18 (δ_H 4.13) and from the aromatic protons H17/18 (δ_H 8.03) to the same aromatic carbon C15 (δ_C 157.0), completing the planar structure of compound 2 (Figure 2A).

Figure 2.

A) Selected 2D NMR correlations of compound 2. (B) (+)-HRESIMS of the fragments detected in a MS/MS experiment of 2.

Again, NOE correlations between aromatic protons H2/6 and H7/8, the methylamide protons H26 and H8, the diastereotopic methylene protons H₂10 and aromatic protons H13/17, and the methylene protons H₂20 and methyl protons H23/24 observed in a ROESY experiment of 2 agreed with the proposed structure (Figure 2A). Furthermore, the fragmentation pathway detected in the (+)-HRESIMS/MS experiment of 2, displayed in Figure 2B, confirmed the proposed planar structure for 2. In this way, the structure of compound 2 was shown to have a framework (tyrosine)–(tyramine)–(3-aminopropanol) similar to that present in aplysamine 4 (3). This compound was also isolated in this study and was identified by comparison of its spectral data with those reported by Scheuer and co-workers for aplysamine 4 (3)¹⁸ isolated from the Verongida sponge Psammaplysilla purpurea.

The S absolute stereochemistry at C8 in both compounds was determined by comparing their [α]_D values (+10.5° for 1 and +47.5° for 2) to those of similar compounds reported previously in the bibliography such as iso-anomoian ([α]_D + 4.5°)¹² and suberedamine A ([α]_D + 19.5°),¹⁰ all containing an l-tyrosine residue. Bromotyrosines bearing a d-tyrosine moiety, such as ianthesine A ([α]_D – 118°) and its degradation tyrosine derivative N,N-dimethyl-d-tyrosine ([α]_D – 73°),²⁵ have been reported to have a negative sign [α]_D value.¹⁵ It is important to notice that both compounds were subjected to high-performance liquid chromatography (HPLC) chiral analysis and just one peak was observed for each compound, ruling out a possible racemization at C8 position during the isolation and purification processes.

Aplyzanzine B (2) was hydrolyzed at 150 °C under acid conditions to afford bromotyrosines (S)-3,5-dibromo-N,N-dimethyltyrosine (4) and (S)-3-(3,5-dibromo-4-methoxyphenyl)-2-(dimethylamino)propanoic acid (5) (Figure 3), whose structures were deduced by NMR spectral data and by (+)-HRESI mass spectra displaying molecular formulae of C₁₂H₁₅Br₂NO₃ and C₁₁H₁₃Br₂NO₃, respectively. The positive [α]_D optical rotation values of +31° and +33° for 4 and 5, respectively, confirmed the same absolute stereochemistry of C8 as established for 2. (R)-3,5-Dibromo-N,N-dimethyltyrosine was obtained previously by acid hydrolysis of ianthesine A,²⁵ whereas compound 5 was reported as an intermediate in the synthesis of several bromotyrosine derivatives.^26,27

Figure 3.

Compounds 4 and 5 obtained by hydrolysis of 2.

Aplyzanzine B (1) and anomoian B (2) exhibit significant cytotoxic activity against three human tumor cell lines from different tissues: A549 (lung carcinoma), HT-29 (colorectal adenocarcinoma), and MDA-MB-231 (breast adenocarcinoma). IC₅₀ values for anomoian B (1) in these cell lines are 5.1, 3.2, and 5.3 μM. IC₅₀ values for aplyzanzine B (2) are 6.1, 1.6, and 7.8 μM. The bromotyrosines 4 and 5 were also evaluated under the same conditions as 2 with the aim of determining a structure–activity relationship for this type of compounds. The lack of cytotoxic activity showed by compounds 4 and 5 seems to indicate that the rest of the molecule plays an important role in the cytotoxic activity of compounds 1 and 2.

In order to get some insights into the mechanism of action of the cytotoxic activity of these compounds, a further study of cell death induction was performed.

The data published by Su et al.¹⁵ indicated that the cytotoxic activity of small bromotyrosine derivatives is mediated through the induction of apoptosis, also known as programmed cell death. During apoptosis, some proteins are cleaved by caspase-3 and they can be used as markers of cell death. Poly (ADP-ribose) polymerase (PARP-1) is a target of caspase-3, and its cleavage is considered to be a hallmark of apoptosis. PARP-1 cleavage can be easily detected by Western blot. In addition, the apoptotic program leads to DNA internucleosomal fragmentation; histone H2AX becomes rapidly phosphorylated when DNA is damaged (known as γH2AX) and plays an important role in the execution of apoptosis. To investigate whether the antiproliferative effect of aplyzanzine B (1) and anomoian B (2) was due to the induction of apoptosis, three human tumor cell lines (the same ones in which cytotoxicity had been previously demonstrated, namely, A549, HT-29, and MDA-MB-231) were incubated with both compounds at 10 and 20 μg/mL for 24 h (also 48 h for A549).

HT-29 was the cell line with higher sensitivity to both compounds at 24 h. Morphological alteration characteristic of apoptosis, such as dense chromatin surrounded by a halo, was observed in the white-field microscopy images (Figure 4). Protein analysis by Western blot confirmed that, in HT-29 cells, both aplyzanzine B (1) and anomoian B (2) exerted their antiproliferative effect through the induction of apoptosis. Increasing concentrations of both compounds led to PARP cleavage and γH2AX induction (Figure 5). It is important to notice that γH2AX induction is not an effect of direct binding to DNA by these compounds. Band-shift assays have shown that, after incubation with these compounds, the electrophoretic migration of a 200 bp DNA fragment is not affected (data not shown).

Figure 4.

Images of human tumor cell lines HT-29, MDA-MB-231, and A-549 under different conditions assayed as indicated in the figure. Images were obtained at 20× magnification with a white-field microscope.

Figure 5.

Detection of PARP and γH2AX by Western blot in HT-29 at 24 h.

We further investigated whether reactive oxygen species (ROS) were involved in the apoptosis induced by aplyzanzine B and anomoian B, as previously established for the dibromotyrosine derivative (1′R,5′S,6′S)-2-(3′,5′-dibromo-1′,6′-dihydroxy-4′-oxacyclohex-2′-enyl) acetonitrile (DT).¹⁵ ROS play a critical role in cellular homeostasis, and their overproduction may induce apoptosis or other types of cell death. ROS scavenger NAC (N-acetylcysteine) was used to pretreat the cells in order to diminish the production of ROS. Pretreatment with 1.5 mM NAC did not avoid PARP cleavage and γH2AX induction, indicating that ROS are not the main mediators of the apoptotic process triggered by these compounds in HT-29 cells.

MDA-MB-231 and A549 cells are more resistant to both compounds under the assay conditions used. In MDA-MB-231 cells, PARP processing is faintly detected at 24 h, as is also the case of γH2AX induction (Figure 6). In A549 cells, incubation for 48 h with both compounds was necessary to detect PARP cleavage and γH2AX induction (Figure 7). ROS scavenger NAC did not affect γH2AX induction in these cell lines.

Figure 6.

Detection of PARP and γH2AX by Western blot in MDA-MB-231 at 24 h.

Figure 7.

Detection of PARP and γH2AX by Western blot in A549 at 48 h.

Previous studies have shown that the psammaplins, also bromotyrosine derivatives, inhibit HDACs.¹⁶ HDAC inhibitors are a promising new class of anticancer agents that act by inhibiting cell proliferation and inducing cell cycle arrest in several cancer cell types. To analyze whether the antiproliferative effect of aplyzanzine B (1) and anomoian B (2) was related to the HDAC inhibition, A549 cells were treated with both compounds at different concentrations. As a consequence of HDAC inhibition, acetylated histone 4 is accumulated in cells. The results, shown in Figure 8, indicated that neither of the compounds inhibited HDACs. Compounds 1 and 2 did not induce acetyl-H4 accumulation as seen for the positive control trichostatin A, a known HDAC inhibitor.

Figure 8.

Acetyl-H4 Western blot in A549 cells treated with different concentrations of aplyzanzine B (1) and anomoian B (2). Trichostatin A was used as a positive control, and tubulin is shown as a loading control.

3. Conclusions

In summary, the new bromotyrosine derivatives anomoian B (1) and aplyzanzine B (2) were isolated from Indonesian sponges and form part of the few examples of bromotyrosine derivatives with a chiral center at C8. Although the oxime group of the α-carbon in tyrosine residues is present in most bromotyrosine derivatives, this group has been replaced in other related analogues by a primary amine as in hexadellin A,^8,20 an N-methylamine group as in anomoian A,^23,27 suberedamines A and B,²⁴ or an N,N′-dimethylamine group as in aplyzanzine A.²⁸

Aplyzanzine B (2) is the first compound of the bromotyrosine family bearing a ketone carbonyl group adjacent to the dibromobenzene ring. Biogenetically, the ketone functionality could arise through the oxidation of the benzylic position of the tyramine unit (see Figure 9). Compounds bearing a OH group at that position have already been reported such as hemifistularin 3,²⁹ aplysamine A,⁹ and fistularin-3.³⁰ A further oxidation of that benzylic OH group could be the origin of the ketone carbonyl group present in 2.

Figure 9.

Biogenesis proposal for the ketone functionality present in aplyzanzine B (2).

The two new compounds described herein have shown moderate antitumor activity against a panel of three human tumor cell lines (A549, HT-29, and MDA-MB-231), being most active in the colon adenocarcinoma HT-29 cell line. Both compounds induce the apoptotic cell death of the tumor cell lines assayed, although none of the mechanisms described for this family of compounds, namely, the induction of ROS and the inhibition of HDACs, seem to be involved in the induction of apoptosis caused by the new compounds.

4. Materials and Methods

4.1. General Experimental Procedures

Optical rotations were determined using a Jasco P-1020 polarimeter. UV spectra were recorded using an Agilent 8453 ultraviolet–visible spectrometer. IR spectra were obtained with a PerkinElmer Spectrum 100 Fourier transform infrared spectrometer with attenuated total reflection sampling. NMR spectra were recorded on a Varian “Unity 500” spectrometer and a Bruker ADVANCE 500 spectrometer at 500/125 MHz (¹H/¹³C). Chemical shifts were reported in parts per million using residual CD₃OH (δ 3.31 ppm for ¹H and 49.0 ppm for ¹³C) as an internal reference. HRESITOFMS was performed on an Agilent 6230 TOF LC/MS chromatograph spectrometer. (+)-ESIMS spectra were recorded using an Agilent 1100 Series LC/MSD spectrometer. All ESIMS/MS measurements were recorded on a QqQ-TOF Applied Biosystems QSTAR Elite. Chiral HPLC analyses were completed in a Chiralpak AD-H column (5 × 150 mm, H₂O + 0.04% CF₃ CO₂ H/CH₃ CN + 0.04% CF₃CO₂H gradient from 20 to 84% in 16 min, flow rate: 3 mL/min).

4.2. Animal Material

The sponge Hexadella cf indica Dendy (47 g) was collected in May 2010 by hand using rebreather diving near Para Island, Indonesia (03°03.603′ N/125°30.496′ E) at a depth of 81 m and was frozen immediately after collection. The two-sponge association of Jaspis sp. and Bubaris sp. (229 g) samples was collected in September 2012 by hand using rebreather diving in Pulau Saujung, Indonesia (07°20.375′ S/117°32.288′ E) at a depth of 100 m and was frozen immediately after collection. Voucher specimens were deposited at PharmaMar.

4.3. Extraction and Isolation

Hexadella cf indica Dendy (47 g) was triturated and exhaustively extracted with CH₃OH/CH₂Cl₂ (1:1, 3 × 400 mL). The combined extracts were concentrated to yield a crude mass of 3.2 g. The crude product was subjected to vacuum liquid chromatography (VLC) on LiChroprep RP-18 with a stepped gradient from H₂O to CH₃OH to CH₂Cl₂. The fraction that eluted with CH₃OH (565 mg) was subjected to HPLC Prep (XBridge Prep C18 5 μm, 19 × 150 mm, gradient H₂O/CH₃CN from 25 to 76% CH₃CN in 11 min and then 100% in 2 min, flow rate: 15 mL/min, UV detection) to yield a crude fraction (263 mg). This fraction was further purified by semipreparative HPLC (SunFire C18 5 μm, 10 × 150 mm, H₂O + 0.1% CF₃CO₂H/CH₃CN + 0.1% CF₃CO₂H gradient from 30 to 70% in 13 min, flow rate: 3 mL/min, UV detection) to obtain anomoian B (1, 17.6 mg, 0.037% wet wt).

A two-sponge association of Jaspis sp. and Bubaris sp. (229 g) was triturated and exhaustively extracted with CH₃OH/CH₂Cl₂ (1:1, 3 × 400 mL). The combined extracts were concentrated to yield a crude mass of 9.7 g. The crude product was subjected to VLC on LiChroprep RP-18 with a stepped gradient from H₂O to CH₃OH to CH₂Cl₂. The fraction that eluted with CH₃OH gave a fraction of 1.88 g, which was subjected to reversed-phase flash chromatography gradient (H₂O/CH₃CN from 5 to 100% in 25 min) to yield a crude fraction (257 mg). This fraction was further purified by semipreparative HPLC (XBridge Prep C18 5 μm, 10 × 250 mm, H₂O + 0.04% CF₃CO₂H/CH₃CN + 0.04% CF₃CO₂H gradient from 20 to 84% in 16 min, flow rate: 3 mL/min, UV detection) to obtain aplyzanzine B (2, 130 mg, 0.057% wet wt). Chiral HPLC analysis of 1 and 2 was performed in a Chiralpak AD-H column (5 × 150 mm, H₂O + 0.04% CF₃CO₂H/CH₃CN + 0.04% CF₃CO₂H gradient from 20 to 84% in 16 min, flow rate: 3 mL/min, MS detection).

4.4. Anomoian B (1)

Colorless amorphous solid; [α]_D + 10.5 (c 0.1, CH₃OH); CD spectrum, see the Supporting Information; IR ν_max: 2929, 1673, 1473, 1261, 1201, 1133, 834, 800, and 722 cm^–1; UV (CH₃OH) λ_max 276 and 284 nm. ¹H NMR (500 MHz) and ¹³C NMR (125 MHz) (see Table 1); HREITOFMS m/z 739.9355 [M + H]⁺ (calcd for C₂₅H₃₄⁷⁹Br₄N₃O₃m/z 739.9328). CD spectrum (MeOH) (see the Supporting Information).

4.5. Aplyzanzine B (2)

Brown amorphous solid; [α]_D + 47.5 (c 0.1, CH₃OH); CD spectrum, see the Supporting Information; IR ν_max: 2972, 1684, 1544, 1474, 1424, 1261, 1200, and 1130 cm^–1; UV (CH₃OH) λ_max 259 nm. ¹H NMR (500 MHz) and ¹³C NMR (125 MHz) (see Table 1); HREITOFMS m/z 767.9285 [M + H]⁺ (calcd for C₂₆H₃₄⁷⁹Br₄N₃O₄m/z 767.9277). CD spectrum (MeOH) (see the Supporting Information).

4.6. Acidic Hydrolysis of Aplyzanzine B

Compound 2 was hydrolyzed in 6 N HCl at 150 °C for 72 h. The hydrolysate was dried under nitrogen and reconstituted in methanol. The residue was purified by HPLC {symmetry C18, 5 μm, 4.6 × 150 mm; linear gradient from 5 to 100% CH₃CN [0.04% trifluoroacetyl (TFA)] in H₂O [0.04% TFA] over 40 min, flow rate: 0.8 mL/min} to give compounds 4 and 5.

4.7. (S)-3,5-Dibromo-N,N-Dimethyltyrosine (4)

Colorless amorphous solid; [α]_D + 30 (c 0.1, CH₃OH) HREITOFMS m/z: 378.9422 [M + H]⁺ (calcd for C₁₂H₁₅⁷⁹Br₂NO₃m/z: 378.9419). ¹H NMR (500 MHz, D₂O): δ 7.53 (s, 2H), 3.88 (s, 3H), 3.73 (dd, J = 9.5, 5.6 Hz, 1H), 3.26 (dd, J = 13.7, 5.6 Hz, 1H), 3.00 (dd, J = 13.7, 9.5 Hz, 1H), 2.88 (s, 6H).

4.8. (S)-3-(3,5-Dibromo-4-methoxyphenyl)-2-(dimethylamino)-propanoic Acid (5)

Colorless amorphous solid; [α]_D + 33 (c 0.1, CH₃OH). HREITOFMS m/z: 365.9355 [M + H]⁺ (calcd for C₁₁H₁₄⁷⁹Br₂NO₃m/z: 365.9335). ¹H NMR (500 MHz, D₂O): δ 7.37 (s, 2H), 3.74 (dd, J = 8.0, 6.3 Hz, 1H), 3.12 (dd, J = 14.4, 6.3 Hz, 1H), 2.99 (dd, J = 14.4, 8.0 Hz, 1H), 2.88 (s, 6H).

4.9. Evaluation of Cytotoxic Activity

The cytotoxic activity of the isolated compounds was evaluated using a panel of three human tumor cell lines: A549 (lung), HT-29 (colon), and MDA-MB-231 (breast). Cells were maintained at 37 °C and 5% CO₂ in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum, 2 mM l-glutamine, and 100 units/mL penicillin–streptomycin. To determine the cell viability, cells were washed twice with phosphate-buffered saline (PBS), fixed for 15 min in 1% glutaraldehyde solution, rinsed twice in PBS, and stained in 0.4% sulforhodamine B solution for 30 min at room temperature. Cells were then rinsed several times with 1% acetic acid solution and were air-dried. SRB was then extracted with a 10 mM Trizma base solution, and the absorbance was measured at 490 nm. GI₅₀ values (concentration of drug that produces a 50% inhibition of cell growth) were calculated using Prism v5.02 software (GraphPad, La Jolla, USA). Experiments were performed in triplicate.

4.10. Western Blotting Analysis

Cell protein extracts were prepared following standard procedures in a radioimmunoprecipitation assay buffer in the presence of protease inhibitors (Complete, Roche Diagnostics) and phosphatase inhibitors (PhosSTOP, Roche Diagnostics). After quantification with the BCA protein assay kit (Thermo Scientific, Waltham, MA, USA), 20 μg of protein was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred onto poly(vinylidene difluoride) membranes (Immobilon-P, Millipore, Billerica, MA, USA). After hybridizing with the appropriated primary [PARP (Santa Cruz), γH2AX and acetyl-histone H4 (Upstate, Millipore), and tubulin (Sigma)] and secondary antibodies (Santa Cruz), blots were developed by a peroxidase reaction using the electrochemical luminescence detection system (Amersham-G.E. Healthcare, Little Chalfont, UK).

Supporting Information Available

The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acsomega.7b00417.

• ¹H, ¹³C, gHSQC, gCOSY, gHMBC, TOCSY, ROESY NMR, and CD spectra of anomoian B (1) and aplyzanzine B (2) and LRESIMS and ¹H NMR spectra of 4 and 5 (PDF)

The authors declare no competing financial interest.