Increased B-cell activating factor (BAFF)/B-lymphocyte stimulator (BLyS) in primary antiphospholipid syndrome is associated with higher adjusted global antiphospholipid syndrome scores

Key messages.

What is already known about this subject?

• BAFF/BLyS is a key cytokine in systemic lupus erythematosus (SLE) and targeting BAFF by belimumab is used to treat patients with SLE.

• Antiphospholipid syndrome (APS) is a major cause of thrombosis and pregnancy morbidity in patients with rheumatic diseases and immunomodulatory treatments are novel drug candidates to treat APS.

What does this study add?

• BAFF levels are elevated in patient with primary APS and correlate with higher adjusted global antiphospholipid syndrome scores.

• In SLE, but not in APS, the expression of BAFF receptors is altered on peripheral blood B cells.

How might this impact on clinical practice?

• A subset of patients with APS may benefit from BAFF- targeting therapies such as belimumab.

Antiphospholipid syndrome (APS) is a systemic autoimmune disease characterised by antiphospholipid antibodies (aPL), thrombosis and obstetric complications. Other manifestations seen in APS include thrombocytopenia, heart valve disease and leg ulcers. APS was first described in patients suffering from systemic lupus erythematosus (SLE). However, APS may also affect patients not suffering from SLE or another underlying disease, termed primary APS (PAPS). Current treatment strategies for APS relying on anticoagulants are suboptimal, particularly for non-thrombotic manifestations and immunomodulatory drugs have been suggested as potential novel drug candidates in APS.^{1 2}

B-cell activating factor (BAFF), also known as B-lymphocyte stimulator (BLyS), is an important growth factor for B cells. The importance of BAFF in SLE is supported by animal models as mice transgenic for BAFF spontaneously develop SLE-like autoimmunity including the production of antibodies against double-stranded DNA (anti-dsDNA).³ In humans, patients with SLE have elevated levels of BAFF which correlate with disease activity.^{4 5} Moreover, belimumab, a monoclonal antibody against BAFF, is approved for the treatment of SLE and several other BAFF-targeting therapies are under development for the treatment of SLE.⁴

In contrast to SLE, little is known on the role of BAFF in APS. Case reports suggest a beneficial effect of belimumab in the treatment of non-thrombotic manifestations of patients with PAPS⁶ and blockade of the BAFF receptor (BAFF-R) prevents the development of APS in a murine model for APS.⁷ Post hoc analyses of the belimumab trials reveal that belimumab reduces anticardiolipin antibodies in patients with SLE.⁸ As a result, belimumab is a promising drug candidate for APS. However, no studies are available that compare the expression of BAFF and its receptors in patients with SLE and APS.

We measured BAFF levels by ELISA in sera of patient with SLE, SLE+APS and PAPS (table 1) and found increased serum levels of BAFF in patients with PAPS compared with healthy controls (HC), similar as in patients with SLE and SLE+APS (all p<0.05, figure 1A). Setting a threshold using the mean plus two SDs of HC, the prevalence of increased BAFF levels in PAPS was 7/29 (24%, p=0.07) and 21/54 (39%, p=0.002) and 17/40 (43%, p=0.001) in patients with SLE and SLE+APS, respectively, as compared with 2/29 (7%) of HC.

Table 1.

Clinical characteristics

	HC (n=29)	PAPS (n=29)	SLE+APS (n=40)	SLE (n=54)
Clinical manifestations
Age	43 (34–50)	40 (33–50)	45 (37–53)	36 (28–48)
Female (%)	93	97	95	96
SELENA-SLEDAI		–	4 (1–5)	4 (2–6)
Malar rash (%)		0	55	65
Discoid rash (%)		0	18	19
Photosensitivity (%)		0	53	43
Oral ulcers (%)		0	35	32
Arthritis (%)		0	65	72
Serositis (%)		0	20	32
Lupus nephritis (%)		0	45	69
Neurologic disorder (%)		10*	18	4
Haematologic disorder (%)		35†	85	67
Arterial thrombosis (%)		59	43	9
Venous thrombosis (%)		38	60	2
Obstetric morbidity (%)		31	23	6
Current drug use
Hydroxychloroquine (%)		21	55	76
Prednisone (%)		0	45	67
Azathioprine (%)		0	41	33
Mycophenolate mofetil (%)		0	10	19
Oral anticoagulant (%)		62	75	0
Aspirin (%)		48	28	20
Serology
Anti-dsDNA (IU/mL)		–	14 (5–58)	27 (6–99)
C3		–	0.79 (0.68–0.92)	0.86 (0.70–1.03)
C4		–	0.14 (0.10–0.22)	0.15 (0.12–0.20)
Lupus anticoagulant (%)		82	65	14
Anticardiolipin IgG (%)		86	78	20
Anticardiolipin IgM (%)		38	15	10
Anti-β2 glycoprotein I IgG (%)		35	26	4
Anti-β2 glycoprotein I IgM (%)		10	5	7
aGAPSS		10 (8–13)	10 (6–11)	–
Hyperlipidaemia (%)		35	24	21
Arterial hypertension (%)		59	66	56

Medians with IQR or percentages of total.

*Seizures

†Thrombocytopenia

aGAPSS, adjusted global antiphospholipid syndrome score; APS, antiphospholipid syndrome; HC, healthy controls; PAPS, primary APS; SELENA, Safety of Estrogens in Lupus Erythematosus National Assessment; SLE, systemic lupus erythematosus; SLEDAI, SLE Disease Activity Index.

Figure 1.

BAFF is increased in primary antiphospholipid syndrome and is associated with higher adjusted global antiphospholipid syndrome scores. (A) Serum levels of BAFF in patients with PAPS as compared with SLE+APS and PAPS. (B) Correlation of serum levels of BAFF with the aGAPSS in patients with PAPS. (C) mRNA expression of BAFF in purified monocytes of patients with PAPS, SLE+APS and SLE. (D) mRNA expression of BAFF-R, TACI en BCMA in purified CD19+ B-cells in patients with PAPS, SLE+APS and PAPS. Abbreviations: aGAPSS, adjusted global antiphospholipid syndrome score; APS, antiphospholipid syndrome; BCMA, B-cell maturation antigen; GUSB, Glucuronidase Beta; HC, healthy controls; ns, not significant; PAPS, primary APS; SLE, systemic lupus erythematosus; TACI, transmembrane activator and CAML interactor.

The adjusted global antiphospholipid syndrome score (aGAPSS) is a validated tool for risk stratification in patients with PAPS⁹ and serum levels of BAFF correlated with higher aGAPSS scores in patients with PAPS (r=0.40, p=0.03) (figure 1B). The aGAPSS includes data regarding cardiovascular risk factors (hypertension and hyperlipidaemia) and aPL status. In this regard, patients with PAPS positive for lupus anticoagulant had higher serum BAFF levels than patients without (p<0.05) whereas there were no significant differences between patients with or without anti-β2 glycoprotein I or anticardiolipin antibodies (data not shown). In patients with SLE, serum levels of BAFF significantly correlated with higher levels of anti-dsDNA antibodies (r=0.39, p<0.001) and lower levels of complement component C3 (r=−0.26, p=0.02, data not shown). Monocytes are major producers of BAFF, therefore we evaluated mRNA expression of BAFF in purified monocytes obtained from patients with SLE, SLE+APS and PAPS and found increased mRNA expression in all three patient groups as compared with HC (all p<0.05, figure 1C).

BAFF is recognised by one of its three receptors which are highly expressed by B cells: BAFF-R, transmembrane activator and CAML interactor (TACI) and B-cell maturation antigen (BCMA). Binding of BAFF to these receptors promotes B-cell survival and maturation through activation of NFκB.⁴ In purified B cells of patients with SLE we found a lower expression of BAFF-R and TACI mRNA, in line with previous studies that assessed protein expression of these receptors on circulating B cells by flow cytometry.^{5 10} However, in patients with APS, both in SLE+APS and PAPS, we found no changes in the mRNA expression of BAFF-R and TACI as compared with HC (all p>0.05, figure 1D). In contrast, patients with SLE and SLE+APS but not patients with PAPS had an increased expression of BCMA compared with HC (all p<0.05, figure 1D). BCMA is predominantly expressed by plasmablasts and plasma cells^{4 10} which are increased in patients with SLE, potentially explaining the difference in BCMA expression between PAPS and SLE. Therefore, the changes in mRNA expression of BAFF-R between patients with SLE, APS and HC may reflect changes in B-cell homeostasis in SLE or APS. Alternatively, alterations in the expression of BAFF-R may reflect internalisation after BAFF binding.¹¹

In conclusion, similar to SLE, both mRNA and serum levels of BAFF are elevated in PAPS, in particular in patients with PAPS with higher aGAPSS which are at higher risk for thrombotic events.⁹ As the treatment response to belimumab in patients with SLE is associated with higher serum levels of BAFF,¹² belimumab might be a therapeutic option in a subset of patients with PAPS with increased BAFF levels.