Figure 1. Targeting BRAF^V600E in tissue-resident macrophages.

(a, b) Breeding scheme for experimental mice and genotype distribution (n=342). (c, d) YFP expression on BM LSK, blood leukocytes and microglia from 1-month-old mice, representative of n=5 per group. (e) Proportion of YFP⁺ F4/80⁺ cells in tissues from 1-month-old mice. Circles represent individual mice. Unpaired two-tailed t-test. (f) A>T transversion encoding BRAF^V600E in YFP⁺ Kupffer cells at the Braf locus. Red and blue bars indicate forward and reverse strands. (g) Ki67 and cleaved Caspase 3 (Casp3) expression in YFP⁺ microglia from 1-month-old brains. n=5 per group. Unpaired two-tailed t-test. (h) GSEA of differentially expressed genes in YFP⁺ microglia from BRAF^VE (n=3) and littermates (n=2) mice. q-value < 0.01. (i) Heatmap representation of selected genes from (h), values are displayed as z-score. See also Extended Data Fig. 1.