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. Author manuscript; available in PMC: 2019 Mar 15.
Published in final edited form as: Methods. 2017 Dec 30;137:11–19. doi: 10.1016/j.ymeth.2017.12.003
DEPC-treated water 35.4 μl
10× Energy mix 20 μl
10 U/μl CPK solution 1.2 μl
2 M KOAc 7 μl
0.1 M Mg(OAc)2 2.4 μl
1 mM amino acids mix 2 μl
RNasin Plus (Promega) 2 μl (80 U)
Total 70 μl