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. 2016 Aug 31;25(4):1169–1174. doi: 10.1007/s10068-016-0186-4

Antioxidant and anti-adipogenic activities of chestnut (Castanea crenata) byproducts

Un-Young Youn 1, Myung-Soo Shon 1, Gyo-Nam Kim 1, Riho Katagiri 2, Kaori Harata 2, Yasuyuki Ishida 2, Seung-Cheol Lee 1,✉
PMCID: PMC6049104  PMID: 30263390

Abstract

The antioxidant and anti-adipogenic activities of chestnut byproducts were evaluated. At 100 μg/mL, the methanol extract (ME) scavenged 34.2% of DPPH and 78.8% of ABTS radicals. The DPPH and ABTS radical scavenging activity of the water extract (WE) was found to be low (13.7 and 33.1%, respectively) compared with controls. WE and ME dose-dependently inhibited lipid accumulation of 3T3-L1 adipocytes. WE and ME at 100 μg/mL suppressed 3T3-L1 adipogenesis by 71.0 and 96.5%, respectively, when compared with mature adipocytes. The results indicated that WE and ME inhibited adipocyte differentiation by down-regulating the mRNA expression levels of CCAAT/enhancer binding protein (C/EBP)-β, C/EBPα, and peroxisome proliferator-activated receptor (PPAR)-γ in 3T3-L1 cells. Our study also revealed that WE and ME inhibited pre- and early stage adipogenesis in 3T3-L1 cells. The results suggest that chestnut byproducts are a promising source of antioxidant and antiobesity molecules.

Keywords: chestnut, byproducts, antioxidant activity, gallic acid

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