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. 2016 Aug 31;25(4):1197–1204. doi: 10.1007/s10068-016-0190-8

Antifungal activity of Azotobacter nigricans against trichothecene-producing Fusarium species associated with cereals

Hanumanthu Nagaraja 1, Gurikar Chennappa 1, Somashekaraiah Rakesh 1, Manjunath Krishnappa Naik 2, Yatgal Sharanappa Amaresh 2, Marikunte Yanjarappa Sreenivasa 1,✉
PMCID: PMC6049108  PMID: 30263394

Abstract

Antifungal efficacy of Azotobacter nigricans on Fusarium infection, total seedlings mass, root and shoot length, and seed germination in maize, sorghum, and wheat were investigated. Antifungal efficacy of A. nigricans against Fusarium sporotrichioides, Fusarium graminearum, Fusarium poae and Fusarium equiseti showed a significant reduction in growth and Fusarium infection incidence (up to 50%) in all the three treated cereals. However, challenge inoculation of Fusarium spp. to the three cereals showed 100% infection incidence. Total mass of the maize seedlings increased two fold by A. nigricans treatment; however, only a slight increase was observed in sorghum and wheat seedlings. The highest vigour index recorded in maize was 1321 against Fusarium crookwellense, 1616.71 against Fusarium sporotrichioides in sorghum, and 1584.8 against Fusarium acuminatum in wheat treated with A. nigricans. Highest germination incidence of 64% was in maize, 67% in sorghum, and 56% in wheat treated with A. nigricans.

Keywords: Azotobacter, biocontrol, cereals, Fusarium, trichothecene

References

  • 1.Foroud NA, Eudes F. Trichothecenes in cereal grains. Int. J. Mol. Sci. 2009;10:147–173. doi: 10.3390/ijms10010147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 2.Yazar S, Omurtag GZ. Fumonisins, trichothecenes and zearalenone in cereals. Int. J. Mol. Sci. 2008;9:2062–2090. doi: 10.3390/ijms9112062. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 3.Wannemacher RW, Wiener SL. Trichothecene mycotoxins. In: Sidell FR, Takafuji ET, Franz D, editors. Medical aspects of chemical and biological warfare. Washington, DC, USA: Office of The Surgeon General at TMM Publications; 1997. pp. 655–676. [Google Scholar]
  • 4.Nayaka SC, Uday Shankar AC, Reddy MS, Niranjana SR, Prakesh HS, Shetty HS, Mortensen CN. Control of Fusarium verticillioides, cause of ear rot of maize, by Pseudomonas fluorescens. Pest Manag. Sci. 2009;65:769–775. doi: 10.1002/ps.1751. [DOI] [PubMed] [Google Scholar]
  • 5.Aiyaz M, Divakara ST, Nayaka SC. Application of beneficial rhizospheric microbes for the mitigation of seed-borne mycotoxigenic fungal infection and mycotoxins in maize. Biocontrol Sci. Techn. 2015;25:1105–1119. doi: 10.1080/09583157.2015.1020760. [DOI] [Google Scholar]
  • 6.Ayala S, Rao EVSP. Perspective of soil fertility management with a focus on fertilizer use for crop productivity. Curr. Sci. India. 2002;82:797–807. [Google Scholar]
  • 7.Joshi KK, Kumar V, Dubey RC, Maheshwari DK. Effect of chemical fertilizer adaptive variants, Pseudomonas aeruginosa GRC2 and Azotobacter chroococcum AC1 on Macrophomena phaseolina causing charcoal rot of Brassica juncea. Korean J. Environ Agric. 2006;25:228–235. doi: 10.5338/KJEA.2006.25.3.228. [DOI] [Google Scholar]
  • 8.Compant S, Duffy B, Nowak J, Clement C, Barka EI. Use of PGPR for biocontrol of plant diseases: Principles, mechanism of action and future prospects. Appl. Environ. Microb. 2005;71:4951–4959. doi: 10.1128/AEM.71.9.4951-4959.2005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 9.Zamanizadeh HR, Hatami N, Aminaee MM, Rakhshandehroo F. Application of biofungicides in control of damping disease off in greenhouse crops as a possible substitute to synthetic fungicides. Int. J. Environ. Sci. Te. 2010;8:129–136. doi: 10.1007/BF03326202. [DOI] [Google Scholar]
  • 10.Dwi S, Siti P, Erman M. Control of Fusarium wilt of chilli with chintnolytic bacteria. Hayati J. Biosci. 2010;17:5–8. doi: 10.4308/hjb.17.1.5. [DOI] [Google Scholar]
  • 11.Cavaglieri L, Orlando J, Rodríguez MI. Biocontrol of Bacillus subtilis against Fusarium verticillioides in vitro and at the maize root level. Res. Microbiol. 2005;156:748–754. doi: 10.1016/j.resmic.2005.03.001. [DOI] [PubMed] [Google Scholar]
  • 12.Nagaraja H, Chennappa G P C, Roa K M, Prasad MP, Sreenivasa MY. Diversity of toxic and phytopathogenic Fusarium species occurring on cereals grown in Karnataka state, India. 3 Biotech. 2016;6:1–8. doi: 10.1007/s13205-016-0399-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 13.Chennappa G, Adkar-Purushothama CR, Suraj U, Tamilvendan K, Sreenivasa MY. Pesticide tolerant Azotobacter isolates from paddy growing areas of northern Karnataka, India. World J. Microb. Biot. 2013;30:1–7. doi: 10.1007/s11274-013-1412-3. [DOI] [PubMed] [Google Scholar]
  • 14.Mathur SB, Kongsdal O. Common laboratory seed health testing methods for detecting fungi. Bassersdorf, Switzerland: International Seed Testing Association; 2003. pp. 89–93. [Google Scholar]
  • 15.Gholami A, Shahsavani S, Nezarat S. The effect of plant growth promoting Rhizobacteria (PGPR) on germination, seedling growth and yield of maize. World Acad. Sci. Eng. Technol. 2009;49:19–24. doi: 10.3923/pjbs.2009.26.32. [DOI] [PubMed] [Google Scholar]
  • 16.Pereira P, Nesci A, Etcheverry MG. Efficacy of bacterial seed treatments for the Control of Fusarium verticillioides in maize. BioControl. 2009;54:103–111. doi: 10.1007/s10526-007-9148-3. [DOI] [Google Scholar]
  • 17.Cavaglieri LR, Passone A, Etcheverry MG. Correlation between screening procedures to select root endophytes for biological control of Fusarium verticillioides in Zea mays. L. Biol. Control. 2004;31:259–267. doi: 10.1016/j.biocontrol.2004.07.006. [DOI] [Google Scholar]
  • 18.Chauhan S, Wadhwa K, Vasudeva M, Narula N. Potential of Azotobacter spp. as biocontrol agents against Rhizoctonia solani and Fusarium oxysporum in cotton (Gossypium hirsutum), guar (Cyamopsis tetragonoloba) and tomato (Lycopersicum esculentum). Arch. Agron. Soil Sci. 2012;58:1365–1385. [Google Scholar]
  • 19.Pereira P, Nesci A, Etcheverry M. Effects of biocontrol agents on Fusarium verticillioides count and fumonisin content in the maize agroecosystem: Impact on rhizospheric bacterial and fungal groups. Biol. Control. 2007;42:281–287. doi: 10.1016/j.biocontrol.2007.05.015. [DOI] [Google Scholar]
  • 20.Sreenivasa MY, Regina SD, Janardhana GR. Survey of postharvest fungi associated with sorghum grains produced in Karnataka (India) J. Plant Prot. Res. 2010;50:335–339. doi: 10.2478/v10045-010-0057-6. [DOI] [Google Scholar]
  • 21.Sreenivasa MY, Dass RS, Janardhana GR. Mycological evaluation of maize grains in Karnataka (India), for post harvest fungal contamination. World Appl. Sci. J. 2011;13:688–692. [Google Scholar]
  • 22.González HHL, Martýnez EJ, Resnik SL. Fungi associated with sorghum grain from Argentina. Mycopathologia. 1997;139:35–41. doi: 10.1023/A:1006803901969. [DOI] [PubMed] [Google Scholar]
  • 23.Burelle KN, Kloepper JW, Reddy MS. Plant growth promoting rhizobacteria as transplant amendments and their effects on indigenous rhizosphere microorganisms. Appl. Soil Ecol. 2006;31:91–100. doi: 10.1016/j.apsoil.2005.03.007. [DOI] [Google Scholar]
  • 24.Klopper JW, Ryu CM. Induced resistance and promotion of plant growth by Bacillus spp. Phytopathology. 2004;94:1259–1266. doi: 10.1094/PHYTO.2004.94.11.1259. [DOI] [PubMed] [Google Scholar]
  • 25.Vessey J. Plant growth promoting rhizobacteria as biofertilizers. Plant Soil. 2003;255:571–586. doi: 10.1023/A:1026037216893. [DOI] [Google Scholar]
  • 26.Biari A, Gholami A, Rahmani HA. Growth promotion and enhanced nutrient uptake of maize (zea mays L.) by application of plant growth promoting rhizobacteria in arid region of Iran. J. Biol. Sci. 2008;8:1015–1020. doi: 10.3923/jbs.2008.1015.1020. [DOI] [Google Scholar]
  • 27.Akhtar MS, Siddiqui ZA. Biocontrol of root rot disease complex of chicken pea by Glomus intradadices, Rhizobium sp. and Pseudomonas straita. Crop Prot. 2008;27:410–417. doi: 10.1016/j.cropro.2007.07.009. [DOI] [Google Scholar]

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