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. 2016 Jun 30;25(3):829–837. doi: 10.1007/s10068-016-0138-z

In vitro culture of Boletus badius as a source of indole compounds and zinc released in artificial digestive juices

Katarzyna Kała 2, Anna Maślanka 1, Katarzyna Sułkowska-Ziaja 2, Jacek Rojowski 1, Włodzimierz Opoka 1, Bożena Muszyńska 2,✉
PMCID: PMC6049148  PMID: 30263342

Abstract

The objective of this study was to obtain the in vitro cultures of Boletus badius under controlled conditions and investigate the release of indole compounds and zinc from the mycelium of B. badius to artificial digestive juices under conditions similar to those in the human gastrointestinal tract. Biomass was obtained from cultures grown using both only the Oddoux medium as well as the same medium with added zinc hydroaspartate and zinc sulfate. The release of 5-hydroxy-L-tryptophan, L-tryptophan, and serotonin from the B. badius biomass extracts to the artificial digestive juices was determined. Differential pulse anodic stripping voltammetry was used to demonstrate that zinc is released from each of the extracted materials. The total amount of zinc in the materials under study was estimated to be between 7.12 and 44.15 mg/100 g dry weight. It was demonstrated that in vitro cultures of B. badius grown using appropriately selected media may supplement zinc and indole compounds.

Keywords: artificial digestive juice, Boletus badius, indole compounds, mycelial culture, zinc supplementation

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