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. 2016 Dec 31;25(6):1627–1631. doi: 10.1007/s10068-016-0250-0

Optimization of ergosterol to vitamin D2 synthesis in Agaricus bisporus powder using ultraviolet-B radiation

Nam Keun Lee 1, Byung-Yong Aan 1,✉
PMCID: PMC6049238  PMID: 30263454

Abstract

Response surface methodology (RSM) was applied to determine the optimum circumstances for conversion of ergosterol to vitamin D2 via ultraviolet-B (UV-B) in white button mushroom (Agaricus bisporus) powder. Three independent variables, namely, exposure time, ambient temperature, and irradiation intensity were investigated using a central composite rotatable design. The RSM ridge analysis determined the following ideal states: exposure time of 10.4 min, ambient temperature of 26.33°C, and UV-B irradiation intensity of 1.36 W/m2. A vitamin D2 content of 741.50±23.75 μg/g (the predicted value was 780.4 μg/g) was obtained under these ideal conditions.

Keywords: Agaricus bisporus, mushroom, vitamin D2, synthesis, ultraviolet irradiation

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