Abstract
To produce onion vinegar with high efficiency, various fermentation conditions, such as varying initial ethanol concentrations and the addition of ethanol or onion juice were optimized. Acetobacter tropicalis KFCC 11476P consumed ethanol at a rate of 0.125–0.140 g/h when the initial ethanol concentrations were 4, 5, and 6%, and the acidity of the fermentation broth reached the maximum level when the ethanol was completely starved. In the case of fed-batch fermentation with continuous feeding, when a small amount of ethanol and onion juice was continuously supplied into the broth after 30 h of fermentation, the acidity continued to increase up to 4.5% at 45 h. All the while, the remaining ethanol content of the fermentation broth was 1.13-1.69%. The maximum acidity of onion vinegar in the pilot–scale fermenter reached 4.6% at 48 h of which fermentation speed was five times faster than the general standard.
Keywords: onion, vinegar, fermentation, acetic acid bacteria, fed–batch
References
- 1.Yukimichi K, Yasuhiro U, Fujiharu Y. The general composition inorganic cations free amino acids and organic acid of special vinegars. Nippon Shokuhin Kogyo Gakk. 1987;34:592–596. doi: 10.3136/nskkk1962.34.9_592. [DOI] [Google Scholar]
- 2.Casale M, Siz AMJ, Gonzlez SJM, Pizarro C, Forina M. Study of the aging and oxidation processes of vinegar samples from different origins during storage by near–infrared spectroscopy. Anal. Chim. Acta. 2006;557:360–366. doi: 10.1016/j.aca.2005.10.063. [DOI] [Google Scholar]
- 3.Kim KJ, Bae YS, Lee SC, Lee WJ, Lee IK, Yoon YK, Park HK, Ha WH. Influence of vinegar–drink with persimmon on oxygen transport function and recovery capacity in exercise. Korean J. Phys. Educ. 1997;36:102–113. [Google Scholar]
- 4.Maselli JA, Horwath RO inventor; Nabisco Brands, Inc., assignee. Combination semi–continuous and batch process for preparation of vinegar. U.S. patent 06,447,047 (1984)
- 5.Lee YC, Lee GY, Kim HC, Park KB, Yoo YJ, Ahn PU, Choi CU, Son SH. Production of high acetic acid vinegar using two stage fermentation. Microbiol. Biotechnol. Lett. 1998;20:663–667. doi: 10.1023/A:1005366406453. [DOI] [Google Scholar]
- 6.Gupta NN, Mehrota RML, Sircar AR. Effect of onion on serum cholesterol blood coagulation factors and fibrinolytic activity in alimentary lipemia. Ind. J. Med. Res. 1966;54:48–54. [Google Scholar]
- 7.Menon IS, Kendal RY, Dewar HA, Newell DJ. Effect of onions on blood fibrinolytic activity. Brit. Med. J. 1968;3:351–352. doi: 10.1136/bmj.3.5614.351. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 8.Huh K, Lee SI, Park JM. Effect of garlic on the hepatic xanthine oxidase activity in rats. Exp. Mol. Med. 1985;18:209–214. [Google Scholar]
- 9.Sheo HJ, Lim HJ, Jung DL. Effects of onion juice on toxicity of lead in rat. J. Korean Soc. Food Sci. Nutr. 1993;22:138–143. [Google Scholar]
- 10.Park PS, Lee BR, Lee MY. Effect of onion diet on carbon tetrachloride toxicity of rats. J. Korean Soc. Food Sci. Nutr. 1991;20:121–125. [Google Scholar]
- 11.Park PS, Lee BR, Lee MY. Effects of onion juice on ethanol–induced hepatic lipid peroxidation in rats. J. Korean Soc. Food Sci. Nutr. 1994;23:750–755. [Google Scholar]
- 12.Park PS, Lee MY. The effect of onion and garlic on copper–phenanthroline complex induced DNA degradation. J. Korean Soc. Food Sci. Nutr. 1992;21:367–371. [Google Scholar]
- 13.Somogyi M. Notes on sugar determination. J. Biol. Chem. 1952;195:19–23. [PubMed] [Google Scholar]
- 14.Kim SW, Park JH, Jun HK. Analysis of optimum condition for production of an onionic vinegar by two–step fermentations. J. Life Sci. 2008;18:1410–1414. doi: 10.5352/JLS.2008.18.10.1410. [DOI] [Google Scholar]
- 15.Shin JS, Lee OS, Jeong YJ. Changes in the components of onion vinegars by two stages fermentation. Korean J. Food Sci. Technol. 2002;34:1079–1084. [Google Scholar]
- 16.Kwak YS. Optimization for the fermentation process of Solanum Nigrum vinegar and its quality evaluation. Seoul, Korea: Seoul National University of Science & Technology; 2015. [Google Scholar]
- 17.Wang F, Hu JH, Guo C, Liu CZ. Enhanced laccase production by Trametes versicolor using corn steep liquor as both nitrogen source and inducer. Bioresource Technol. 2014;166:602–605. doi: 10.1016/j.biortech.2014.05.068. [DOI] [PubMed] [Google Scholar]
- 18.Maddipati P, Atiyeh HK, Bellmer DD, Huhnke RL. Ethanol production from syngas by Clostridium strain P11 using corn steep liquor as a nutrient replacement to yeast extract. Bioresource Technol. 2011;102:6494–6501. doi: 10.1016/j.biortech.2011.03.047. [DOI] [PubMed] [Google Scholar]
- 19.Qi Z, Yang H, Xia X, Quan W, Wang W, Yu X. Achieving high strength vinegar fermentation via regulating cellular growth status and aeration strategy. Process Biochem. 2014;49:1063–1070. doi: 10.1016/j.procbio.2014.03.018. [DOI] [Google Scholar]
- 20.Kim MY. Effect of inoculation level of starter culture (Acetobacter aceti) and acetic acid addition on fermentation of Makgeolli vinegar. Seoul, Korea: Konkuk University; 2011. [Google Scholar]
- 21.Park JH. Studies on the production of an onion vinegar by two–stage fermentation. Pusan, Korea: Pusan National University; 2007. [Google Scholar]
- 22.Ito T, Sota H, Honda H, Shimizu K, Kobayashi T. Efficient acetic acid production by repeated fed–batch fermentation using two fermentors. Appl. Microbiol. Biot. 1991;36:295–299. doi: 10.1007/BF00208144. [DOI] [Google Scholar]
- 23.Qi Z, Yang H, Xia X, Xin Y, Zhang L, Wang W, Yu X. A protocol for optimization vinegar fermentation according to the ration of oxygen consumption versus acid yield. J. Food Eng. 2013;116:304–309. doi: 10.1016/j.jfoodeng.2012.12.029. [DOI] [Google Scholar]
- 24.Jeong YJ. Production of beverages and fruits vinegar using Kyungpook special products (persimmom, apple and grape) Food Ind. Nutr. 2000;5:53–59. [Google Scholar]