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. 2016 Oct 31;25(5):1501–1505. doi: 10.1007/s10068-016-0233-1

Highly efficient and specific separation of Staphylococcus aureus from lettuce and milk using Dynabeads protein G conjugates

Shuai Wei 1, Byung-Jae Park 1, Kun-Ho Seo 2, Deog-Hwan Oh 1,✉
PMCID: PMC6049271  PMID: 30263437

Abstract

An immunomagnetic separation method using antibody-coated Dynabeads® Protein G was developed for specific and efficient separation of Staphylococcus aureus in lettuce and whole milk. The amount of immunomagnetic beads (IMBs) and conjugation conditions were optimized. A high capture efficiency was obtained with 0.4 mg of IMBs, an immunoreaction time of 20 min, and a separation time of 1 min without wash. Under optimal conditions, the capture efficiency (CE) for 100-105 CFU/mL of S. aureus was higher than 91.46%. The IMBs showed high specificity even with a high constant number (107 CFU/mL) of Bacillus cereus, Micrococcus luteus, and Lactobacillus plantarum. The CE of IMBs against S. aureus at concentrations from 102 to 105 CFU/mL ranged from 78.70 to 94.77% for lettuce and 60.0 to 73.27% for milk samples. This IMS can be an appropriate selection for combining with bacterial detection method or efficient isolation procedure for S. aureus from foods.

Keywords: Staphylococcus aureus, immunomagnetic separation, Dynabeads® Protein G

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