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. 2016 Feb 29;25(1):159–164. doi: 10.1007/s10068-016-0024-8

Enzyme and high pressure assisted extraction of tricin from rice hull and biological activities of rice hull extract

Chae-Young Park 1, Sohyun Kim 1, Dabeen Lee 1, Dong June Park 2, Jee-Young Imm 1,✉
PMCID: PMC6049346  PMID: 30263252

Abstract

Enzymatic hydrolysis was performed in conjunction with high hydrostatic pressure (HPP) treatment to extract tricin from rice hull. Enzymatic hydrolysis performed with Celluclast (0.5%, w/w) prior to HPP (500 MPa) treatment yielded maximum tricin content (32.9 mg/kg rice hull). The tricin content obtained by conventional solvent extraction and HPP treatment alone were 14.7 and 19.7 mg/kg rice hull, respectively. The rice hull extract containing tricin displayed antioxidant, anti-inflammatory, and antiadipogenic activities. The efficacy of rice hull extract obtained by either HPP or combination of enzymatic hydrolysis and HPP treatments was significantly greater than that of extract prepared by traditional solvent extraction.

Keywords: rice hull, tricin, enzymatic hydrolysis, high hydrolytic pressure, biological activities

References

  • 1.Lim JS, Manan ZA, Alwi SRW, Hashim H. A review on utilisation of biomass from rice industry as a source of renewable energy. Renew. Sust. Energ. Rev. 2012;16:3084–3094. doi: 10.1016/j.rser.2012.02.051. [DOI] [Google Scholar]
  • 2.Deng GF, Xu XR, Zhang Y, Li D, Gan RY, Li HB. Phenolic compounds and bioactivities of pigmented rice. Crit. Rev. Food Sci. 2013;53:296–306. doi: 10.1080/10408398.2010.529624. [DOI] [PubMed] [Google Scholar]
  • 3.Devi RR, Arumughan C. Phytochemical characterization of defatted rice bran and optimization of a process for their extraction and enrichment. Bioresource Technol. 2007;98:3037–3043. doi: 10.1016/j.biortech.2006.10.009. [DOI] [PubMed] [Google Scholar]
  • 4.Durate-Almeida JM, Novoa AV, Linares AF, Lajolo FM, Genovese MI. Antioxidant activity of phenolics compounds from sugar cane (Saccharumofficinarum L.) juice. Plant Food Hum. Nutr. 2006;61:187–192. doi: 10.1007/s11130-006-0032-6. [DOI] [PubMed] [Google Scholar]
  • 5.Wang HB, Yao H, Bao GH, Zhang HP, Qin GW. Flavone glycosides with immunomodulatory activity from the leaves of Pleioblastus amarus. Phytochemistr. 2004;65:969–974. doi: 10.1016/j.phytochem.2003.11.013. [DOI] [PubMed] [Google Scholar]
  • 6.Cai H, Hudson EA, Mann P, Verschoyle RD, Greaves P, Manson MM, Steward WP, Gescher AJ. Growth inhibitory and cell cycle arresting properties of the rice bran constituent tricin in human-derived breast cancer cells in vitro and in nude mice in vivo. Brit. J. Cance. 2004;91:1364–1371. doi: 10.1038/sj.bjc.6602124. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 7.Lee D, Park HY, Kim S, Park Y, Bang MH, Imm JY. Antiadipogenic effect of oat hull extract conataing tricin on 3T3-L1 adipocytes. 2015. [Google Scholar]
  • 8.Moheb A, Grondin M, Ibrahim RK, Roy R, Sarhan F. Winter wheat hull (husk) is a valuable source for tricin, a potential selective cytotoxic agent. Food Chem. 2013;138:931–937. doi: 10.1016/j.foodchem.2012.09.129. [DOI] [PubMed] [Google Scholar]
  • 9.Mohanlal S, Parvathy R, Shalini V, Helen A, Jayalekshmy A. Isolation, characterization and quantification of tricin and flavonolignans in the medicinal rice Njavara (Oryza sativa L.) as compared to staple varieties. Plant Food Hum. Nutr. 2011;66:91–96. doi: 10.1007/s11130-011-0217-5. [DOI] [PubMed] [Google Scholar]
  • 10.Strati IF, Gogou E, Oreopoulou V. Enzyme and high pressure assisted extraction of carotenoids from tomato waste. Food Bioprod. Process. 2015;94:668–674. doi: 10.1016/j.fbp.2014.09.012. [DOI] [Google Scholar]
  • 11.Xi J, Shen D, Li Y, Zhang R. Miromechanism of ultrahigh pressure extraction of active ingredients from green tea leaves. Food Contro. 2011;22:1473–1476. doi: 10.1016/j.foodcont.2011.03.008. [DOI] [Google Scholar]
  • 12.Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Method Enzymol. 1999;299:152–178. doi: 10.1016/S0076-6879(99)99017-1. [DOI] [Google Scholar]
  • 13.Kim DO, Jeong SW, Lee CY. Antioxidant capacity of phenolic phytochemicals from various cultivars of plums. Food Chem. 2003;81:321–326. doi: 10.1016/S0308-8146(02)00423-5. [DOI] [Google Scholar]
  • 14.Lee JH, Jeon JK, Kim SG, Kim SH, Chun T, Imm JY. Comparative analyses of total phenols, flavonoids, saponins and antioxidant activity in yellow soy beans and mung beans. Int. J. Food Sci. Tech. 2011;46:2513–2519. doi: 10.1111/j.1365-2621.2011.02775.x. [DOI] [Google Scholar]
  • 15.Marcocci L, Maguire JJ, Droylefaix MT, Packer L. The nitric oxide-scavenging properties of ginko biloba extract EGb 761. Biochem. Bioph. Res. Co. 1994;201:748–755. doi: 10.1006/bbrc.1994.1764. [DOI] [PubMed] [Google Scholar]
  • 16.Landbo AK, Meyer AS. Enzyme-assisted extraction of antioxidative phenols from black currant juice press residues (Ribes nigum) J. Agr. Food Chem. 2001;49:3169–3177. doi: 10.1021/jf001443p. [DOI] [PubMed] [Google Scholar]
  • 17.Gottschalk LF, Oliveira RA, da Silva Bon EP. Cellulase, xylanases, β-glucosidase and ferulic acid esterase produced by Trichoderma and Aspergillus act synergistically in the hydrolysis of sugarcane bagasse. Biochem. Eng. J. 2010;51:72–78. doi: 10.1016/j.bej.2010.05.003. [DOI] [Google Scholar]
  • 18.Fu YJ, Liu W, Zu YG, Tong MH, Li SM, Yan MM, Efferth T, Luo H. Enzyme assisted extraction of luteolin and apigenin from pigeonpea [Cajanus cajan (L.) Millsp.] leaves. Food Chem. 2008;111:508–512. doi: 10.1016/j.foodchem.2008.04.003. [DOI] [PubMed] [Google Scholar]
  • 19.Wu M, Pang J, Lu F, Zhang X, Che L, Xu F, Sun R. Application of new expansion pretreatment method on agricultural waste. Part I: Influence of pretreatment on the properties of lignin. Ind. Crop. Prod. 2013;50:887–895. doi: 10.1016/j.indcrop.2013.08.047. [DOI] [Google Scholar]
  • 20.Butsat S, Siriamornpun S. Antioxidant capacities and phenolic compounds of the husk, bran and endosperm of Thai rice. Food Chem. 2010;119:606–613. doi: 10.1016/j.foodchem.2009.07.001. [DOI] [Google Scholar]
  • 21.Patras A, Brunton NP, Pieve SD, Butler F. Impact of high pressure processing on total antioxidant activity, phenolic, ascorbic acid, anthocyanin content and color of strawberry and blackberry purees. Innov. Food Sci. Emerg. 2009;10:308–313. doi: 10.1016/j.ifset.2008.12.004. [DOI] [Google Scholar]
  • 22.Xi J. Caffeine extraction from green tea leaves assisted by high pressure processing. J. Food Eng. 2009;94:105–109. doi: 10.1016/j.jfoodeng.2009.03.003. [DOI] [Google Scholar]
  • 23.Rivalain N, Roquain J, Demazeau G. Development of high hydrostatic pressure in biosciences: Pressure effect on biological structure and potential applications in biotechnologies. Biotechnol. Adv. 2010;28:659–672. doi: 10.1016/j.biotechadv.2010.04.001. [DOI] [PubMed] [Google Scholar]
  • 24.Prasad KN, Yang B, Zhao M, Wei X, Jiang Y, Chen F. High pressure extraction of corilagin from longan (Dimocarpus longan Lour.) fruit pericarp. Sep. Purif. Technol. 2009;70:41–45. doi: 10.1016/j.seppur.2009.08.009. [DOI] [Google Scholar]
  • 25.Kim DJ, Oh SK, Chun A, Yoon MR, Hong HC, Choi IS, Lee JS, Yu KW, Kim YK. Evaluation of biological activities of rice husk extracts. J. Food Sci. Nutr. 2011;16:179–183. doi: 10.3746/jfn.2011.16.2.179. [DOI] [Google Scholar]
  • 26.Verschoyle RD, Greaves P, Cai H, Borkhardt A, Broggini M, D’Incalci M, Riccio E, Doppalapudi R, Kapetanovic IM, Steward WP. Preliminary safety evaluation of the putative cancer chemopreventive agent tricin, a naturally occurring flavone. Cancer Chemoth. Pharm. 2006;57:1–6. doi: 10.1007/s00280-005-0039-y. [DOI] [PubMed] [Google Scholar]
  • 27.Zamora R, Vodovotz Y, Billar TR. Inducible nitric oxide synthase and inflammatory diseases. Mol. Med. 2000;6:347–373. [PMC free article] [PubMed] [Google Scholar]
  • 28.Chung IM, Hahn SJ, Ahmad A. Confirmation of potential herbicidal agents in hulls of rice, Oryza sativa. J. Chem. Ecol. 2005;31:1339–1352. doi: 10.1007/s10886-005-5290-5. [DOI] [PubMed] [Google Scholar]
  • 29.Nenadis N, Kyriakoudi A, Tsimidou MZ. Impact of alkaline or acid digestion to antioxidant activity, phenolic content and composition of rice hull extracts. LWT-Food Sci. Technol. 2013;54:207–215. doi: 10.1016/j.lwt.2013.05.005. [DOI] [Google Scholar]
  • 30.Shalini V, Bhaskar S, Kumar KS, Mohanlal S, Jayalekshmy A, Helen A. Molecular mechanisms of anti-inflammatory action of the flavonoid, tricin from Njavara rice (Oryza sativa L.) in human peripheral blood mononuclear cells: Possible role in the inflammatory signaling. Int. Immunopharmacol. 2012;14:32–38. doi: 10.1016/j.intimp.2012.06.005. [DOI] [PubMed] [Google Scholar]
  • 31.Jung YS, Kim DH, Hwang JY, Yun NY, Lee YH, Han SB, Hwang BY, Lee MS, Jeong HS, Hong JT. Anti-inflammatory effect of tricin 4'-O-(threo-β-guaiacylglyceryl) ether, a novel flavonolignan compound isolated from Njavara on in RAW 264.7 cells and in ear mice edema. Toxicol. Appl. Pharm. 2014;277:67–76. doi: 10.1016/j.taap.2014.03.001. [DOI] [PubMed] [Google Scholar]
  • 32.Yun JW. Possible anti-obesity therapeutics from nature-A review. Phytochemistr. 2010;71:1625–1641. doi: 10.1016/j.phytochem.2010.07.011. [DOI] [PubMed] [Google Scholar]
  • 33.Ono M, Fujimori K. Antiadipogenic effect of dietary apigenin through activation of AMPK in 3T3-L1 cells. J. Agr. Food Chem. 2011;59:13346–13352. doi: 10.1021/jf203490a. [DOI] [PubMed] [Google Scholar]
  • 34.Lee J, Jung E, Lee J, Kim S, Huh S, Kim Y, Kim Y, Byun SY, Kim YS, Park D. Isorhamnetin represses adipogenesis in 3T3L1 Cells. Obesit. 2009;17:226–232. doi: 10.1038/oby.2008.472. [DOI] [PubMed] [Google Scholar]

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