Abstract
Optimal drying techniques for maintaining high levels of bioactive compounds and antioxidant activities in black chokeberries were investigated. Effects of 3 drying methods on total bioactive compound contents and in vitro antioxidant activities in 80% ethanol extracts were evaluated. Fresh black chokeberries were dried using sun-drying, freeze-drying, and oven-drying. Highest amounts of total polyphenols, flavonoids, and anthocyanins were detected in freeze-dried black chokeberry extracts after sun and oven-drying. 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS), and superoxide anion scavenging activities in black chokeberry extracts were also evaluated. Freeze-dried berries produced strongest antioxidant activities. Freeze-drying was the optimal drying method for maintaining high levels of bioactive compounds in 80% ethanol extracts of dried black chokeberries.
Keywords: Aronia melanocarpa, black chokeberry, polyphenol, flavonoid, anthocyanin
References
- 1.Slimestad R, Torskangerpoll K, Nateland HS, Johannessen T, Giske NH. Flavonoids from black chokeberries, Aronia melanocarpa. J. Food Compos. Anal. 2005;18:61–68. doi: 10.1016/j.jfca.2003.12.003. [DOI] [Google Scholar]
- 2.Jakobek L, Šeruga M, Novak I, Medvidovic-Kosanovic M. Flavonols, phenolic acids and antioxidant activity of some red fruits. Deut. Lebensm.-rundsch. 2007;103:369–378. [Google Scholar]
- 3.Kähkönen MP, Hopia AI, Vuorela HJ, Rauha J, Pihlaja K, Kujala TS, Heinonen M. Antioxidant activity of plant extracts containing phenolic compounds. J. Agr. Food Chem. 1999;47:3954–3962. doi: 10.1021/jf990146l. [DOI] [PubMed] [Google Scholar]
- 4.Carvalho IS, Cavaco T, Brodelius M. Phenolic composition and antioxidant capacity of six Artemisia species. Ind. Crop. Prod. 2011;33:382–388. doi: 10.1016/j.indcrop.2010.11.005. [DOI] [Google Scholar]
- 5.Hellström JK, Shikov AN, Makarova MN, Pihlanto AM, Pozharitskaya ON, Ryhänen E, Kivijärvi P, Makarov VG. Blood pressure-lowering properties of chokeberry (Aronia mitchurinii, var. Viking) J. Funct. Food. 2010;2:163–169. doi: 10.1016/j.jff.2010.04.004. [DOI] [Google Scholar]
- 6.Kedzierska M, Olas B, Wachowicz B, Glowacki R, Bald E, Czernek U, Szydlowska-Pazera K, Potemsk P. Effect of the commercial extract of aronia on oxidative stress in blood platelets isolated from breast cancer patients after the surgery and various phases of the chemotherapy. Fitoterapi. 2012;83:310–317. doi: 10.1016/j.fitote.2011.11.007. [DOI] [PubMed] [Google Scholar]
- 7.Oszmiañski J, Wojdylo A. Aronia melanocarpa phenolics and their antioxidant activity. Eur. Food Res. Technol. 2005;221:809–813. doi: 10.1007/s00217-005-0002-5. [DOI] [Google Scholar]
- 8.Ahrné LM, Pereira NR, Staack N, Floberg P. Microwave convective drying of plant foods at constant and variable microwave power. Dry. Technol. 2007;25:1149–1153. doi: 10.1080/07373930701438436. [DOI] [Google Scholar]
- 9.Stojanovic J, Silva JL. Influence of osmotic concentration, continuous high frequency ultrasound and dehydration on antioxidants, colour and chemical properties of rabbiteye blueberries. Food Chem. 2007;101:898–906. doi: 10.1016/j.foodchem.2006.02.044. [DOI] [Google Scholar]
- 10.AOAC. Official Method of Analysis of AOAC Intl. 1995. pp. 1–26. [Google Scholar]
- 11.Zhou K, Su L, Yu L. Phytochemicals and antioxidant properties in wheat bran. J. Agr. Food Chem. 2004;52:6108–6114. doi: 10.1021/jf049214g. [DOI] [PubMed] [Google Scholar]
- 12.Woisky R, Salatino A. Analysis of propolis: Some parameters and procedures for chemical quality control. J. Apic. Sci. 1998;37:99–105. [Google Scholar]
- 13.Cheung LM, Cheung PC, Ooi VE. Antioxidant activity and total phenolics of edible mushroom extracts. Food Chem. 2003;81:249–255. doi: 10.1016/S0308-8146(02)00419-3. [DOI] [Google Scholar]
- 14.Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Bio. Med. 1999;26:1231–1237. doi: 10.1016/S0891-5849(98)00315-3. [DOI] [PubMed] [Google Scholar]
- 15.Wang J, Yuan X, Jin Z, Tian Y, Song H. Free radical and reactive oxygen species scavenigng activities of peanut skins extract. Food Chem. 2007;104:242–250. doi: 10.1016/j.foodchem.2006.11.035. [DOI] [Google Scholar]
- 16.Oyaizu M. Studies on product of browning reaction prepared from glucose amine. Jap. J. Nutr. 1986;44:307–315. doi: 10.5264/eiyogakuzashi.44.307. [DOI] [Google Scholar]
- 17.Drewnowski A, Gomez-Carneros C. Bitter taste, phytonutrients, and the consumer: A review. Am. J. Clin. Nutr. 2000;72:1424–1435. doi: 10.1093/ajcn/72.6.1424. [DOI] [PubMed] [Google Scholar]
- 18.Jakobek L, Drenjanèeviæ M, Jukiæ V, Šeruga M. Phenolic acids, flavonols, anthocyanins and antiradical activity of “Nero”, “Viking”, “Galicianka” and wild chokeberries. Sci. Hortic.-Amsterda. 2012;147:56–63. doi: 10.1016/j.scienta.2012.09.006. [DOI] [Google Scholar]
- 19.Benvenuti S, Pellati F, Melegari M, Bertelli D. Polyphenols, anthocyanins, ascorbic acid, and radical scavenging activity of Rubus, Ribes, and Aronia. J. Food Sci. 2004;69:164–169. [Google Scholar]
- 20.Oboh G. Effect of blanching on the antioxidant properties of some tropical green leafy vegetables. LWT-Food Sci. Technol. 2005;38:513–517. doi: 10.1016/j.lwt.2004.07.007. [DOI] [Google Scholar]
- 21.Valcheva-Kuzmanova S, Borisova P. Hepatoprotective effect of the natural fruit juice from Aronia melanocarpa on carbon tetrachloride-induced acute liver damage in rats. Exp. Toxicol. Pathol. 2004;56:195–201. doi: 10.1016/j.etp.2004.04.012. [DOI] [PubMed] [Google Scholar]
- 22.Pantelidis GE, Vasilakakis M, Manganaris GA, Diamantidis Gr. Antioxidant capacity, phenol, anthocyanin and ascorbic acid contents in raspberries, black berries, red currants, gooseberries and Cornelian cherries. Food Chem. 2007;102:777–783. doi: 10.1016/j.foodchem.2006.06.021. [DOI] [Google Scholar]
- 23.Hsu C-L, Chen W, Weng Y-M, Tseng C-Y. Chemical composition, physical properties and antioxidant activities of yam flours as affected by different drying methods. Food Chem. 2003;83:85–92. doi: 10.1016/S0308-8146(03)00053-0. [DOI] [Google Scholar]
- 24.Lim YY, Murtijaya J. Antioxidant properties of Phyllanthus amarus extracts as affected by different drying methods. LWT-Food Sci. Technol. 2007;40:1664–1669. doi: 10.1016/j.lwt.2006.12.013. [DOI] [Google Scholar]
- 25.Laguerre JC, Tauzin V, Grenier E. Hot air and microwave drying of onions: A comparative study. Dry. Technol. 1999;17:1471–1480. doi: 10.1080/07373939908917629. [DOI] [Google Scholar]
- 26.Hossain MB, Barry-Ryan C, Martin-Diana AB, Brunton NP. Effect of drying method on the antioxidant capacity of six Lamiceae herbs. Food Chem. 2010;123:85–91. doi: 10.1016/j.foodchem.2010.04.003. [DOI] [Google Scholar]
- 27.Chan EWC, Lim YY, Wong SK, Lim KK, Tan SP, Lianto FS, Yong MY. Effects of different drying methods on the antioxidant properties of leaves and tea of ginger species. Food Chem. 2009;113:166–172. doi: 10.1016/j.foodchem.2008.07.090. [DOI] [Google Scholar]
- 28.Yousif AL, Scaman CH, Durance TD, Girard B. Flavor volatiles and physical properties of vacuum-microwave and airdried sweet basil (Ocimum basilicum L.) J. Agr. Food Chem. 1999;47:4777–4781. doi: 10.1021/jf990484m. [DOI] [PubMed] [Google Scholar]
- 29.Ratti C. Hot air and freeze-drying of high-value foods: A review. J. Food Eng. 2001;49:311–319. doi: 10.1016/S0260-8774(00)00228-4. [DOI] [Google Scholar]
- 30.Borchani C, Besbes S, Masmoudi M, Blecker C, Paquot M, Attia H. Effect of drying methods on physico-chemical and antioxidant properties of date fibre concentrates. Food Chem. 2011;125:194–1201. doi: 10.1016/j.foodchem.2010.10.030. [DOI] [Google Scholar]
- 31.Ma L, Chen H, Zhu W, Wang Z. Effect of different drying methods on physicochemical properties and antioxidant activities of polysaccharides extracted from mushroom Inonotus obliquus. Food Res. Int. 2013;50:633–640. doi: 10.1016/j.foodres.2011.05.005. [DOI] [Google Scholar]