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. 2016 Feb 29;25(1):185–192. doi: 10.1007/s10068-016-0028-4

Optimization of submerged Aspergillus oryzae S2 α-amylase production

Belgacem Naili 1, Mouna Sahnoun 1,✉, Samir Bejar 1, Radhouane Kammoun 1
PMCID: PMC6049366  PMID: 30263256

Abstract

Use of 4 agro-industrial by products and organic materials as nitrogen sources for production of Aspergillus oryzae S2 α-amylase in liquid culture was investigated. The 2 agro-industrial byproducts maltose and saccharose, and also lactose and starch were individually evaluated for use as carbon sources. A Box-Behnken experimental design was used to determine optimal conditions for production of α-amylase. A maximum amylase activity of 750 U/mL was obtained at a temperature of 24°C, a urea concentration of 1 g/L, and a C/N ratio of 2. Laboratory scale application of optimal conditions in a 7 L fermentor produced a final α-amylase activity of 770 U/mL after 3 days of batch cultivation. Addition of 10% starch to the culture medium each 12 h immediately after the stationary phase of cell growth led to a production yield of 1,220 U/mL at the end of fed-batch cultivation.

Keywords: Aspergillus oryzae strain S2, Box–Behnken design, 7 L-fermentor, batch, fed-batch

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