Abstract
Beetroot is a vegetable rich in nitrate (NO−3), antioxidants and phenolic compounds that are related to improvements in cardiovascular function and exercise performance. However, it is unknown if convenient forms of beetroot administration provide different amounts of these nutrients. The total antioxidant potential (TAP), total phenolic (TPC), sugar, organic acid, and NO−3 contents of beetroot juice (BJ), chips (BC), powder (BP), and cooked beetroot (CB) were compared. Significant (p<0.01) differences in chemical compositions and functional properties were found between beetroot formulations. Higher amounts of TAP and organic acids were observed in BC and BP, compared with the other formulations. BJ exhibited the highest contents of total sugars, TPC, and NO−3. All beetroot formulations were suitable and advantageous based on taste preferences and convenience for consumers and for nutrient amounts required to meet dietary recommendations.
Keywords: vegetable, antioxidant, beetroot, drying method
References
- 1.Liu RH. Health-promoting components of fruits and vegetables in the diet. Adv. Nutr. 2013;4:384–392. doi: 10.3945/an.112.003517. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 2.Boeing H, Bechthold A, Bub A, Ellinger S, Haller D, Kroke A, Leschik-Bonnet E M M, Oberritter H, Schulze M, Stehle P, Watzl B. Critical review: Vegetables and fruit in the prevention of chronic diseases. Eur. J. Nutr. 2012;51:637–663. doi: 10.1007/s00394-012-0380-y. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 3.Hord NG, Tang Y, Bryan NS. Food sources of nitrates and nitrites: The physiologic context for potential health benefits. Am. J. Clin. Nutr. 2009;90:1–10. doi: 10.3945/ajcn.2008.27131. [DOI] [PubMed] [Google Scholar]
- 4.Lange W, Brandenburg WA, De bock TS. Taxonomy and cultonomy of beet (Beta vulgaris L.) Bot. J. Linn. Soc. 1999;130:81–96. doi: 10.1111/j.1095-8339.1999.tb00785.x. [DOI] [Google Scholar]
- 5.United State Department of Agriculture (USDA). National Nutrient Database for Standard Reference. Available from: http://ndb.nal.usda.gov/ndb/foods/list. Acessed Aug. 10, 2013.
- 6.Koubaier HBH, Snoussi A, Essaidi I, Chaabouni MM, Thonart P, Bouzouita N. Betalain and phenolic compositions, antioxidant activity of tunisian red beet (Beta vulgaris L. conditiva) roots and stems extracts. Int. J. Food Prop. 2014;17:1934–1945. doi: 10.1080/10942912.2013.772196. [DOI] [Google Scholar]
- 7.Wootton-Beard PC, Ryan L. Combined use of multiple methodologies for the measurement of total antioxidant capacity in UK commercially available vegetable juices. Plant Food. Hum. Nutr. 2012;67:142–147. doi: 10.1007/s11130-012-0287-z. [DOI] [PubMed] [Google Scholar]
- 8.Kujala TS, Loponen JM, Klika KD, Pihlaja K. Phenolics and betacyanins in red beetroot (Beta vulgaris) root: Distribution and effect of cold storage on the content of total phenolics and three individual compounds. J. Agr. Food Chem. 2000;48:5338–5342. doi: 10.1021/jf000523q. [DOI] [PubMed] [Google Scholar]
- 9.Georgiev VG, Weber J, Kneschke EM, Denev PN, Bley T, Pavlov AI. Antioxidant activity and phenolic content of betalain extracts from intact plants and hairy root cultures of the red beetroot Beta vulgaris cv. detroit dark red. Plant Food. Hum. Nutr. 2010;65:105–111. doi: 10.1007/s11130-010-0156-6. [DOI] [PubMed] [Google Scholar]
- 10.Kapil V, Webb AJ, Ahluwalia A. Inorganic nitrate and the cardiovascular system. Hear. 2010;96:1703–1709. doi: 10.1136/hrt.2009.180372. [DOI] [PubMed] [Google Scholar]
- 11.Stokes KY, Dugas TR, Tang Y, Garg H, Guidry E, Bryan NS. Dietary nitrite prevents hypercholesterolemic microvascular inflammation and reverses endothelial dysfunction. Endocrinol. Metab. 2009;296:1281–1288. doi: 10.1152/ajpheart.01291.2008. [DOI] [PubMed] [Google Scholar]
- 12.Murph M, Eliot K, Heuertz RM, Weiss E. Whole beetroot consumption acutely improves running performance. J. Acad. Nutr. Diet. 2012;112:548–552. doi: 10.1016/j.jand.2011.12.002. [DOI] [PubMed] [Google Scholar]
- 13.Vanhatalo A, Fulford J, Bailey SJ, Blackwell JR, Winyard PG, Jones AM. Dietary nitrate reduces muscle metabolic perturbation and improves exercise tolerance in hypoxia. J. Physiol.-Londo. 2011;15:5517–5528. doi: 10.1113/jphysiol.2011.216341. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 14.Glód BK, Piszcz P, Czajka K, Zarzycki PK. A New Total antioxidant potential measurements using rp-hplc assay with fluorescence detection. J. Chromatogr. Sci. 2011;49:401–404. doi: 10.1093/chromsci/49.5.401. [DOI] [PubMed] [Google Scholar]
- 15.Wantusiak PM, Glód BK. Application of UV detection in HPLC in the total antioxidant potential assay. Cent. Eur. J. Chem. 2012;10:1786–1790. [Google Scholar]
- 16.Sánchez-Rangel JC, Benavides J, Heredia JB, Cisneros-Zevallos L, Jacobo-Velázquez DA. The Folin-Ciocalteu assay revisited improvement of its specificity for total phenolic content determination. Anal. Method. 2013;5:5990–5999. doi: 10.1039/c3ay41125g. [DOI] [Google Scholar]
- 17.Mohdaly AAA, Hassanien MFR, Mahmoud A, Sarhan MA, Smetanska I. Phenolics extracted from potato, sugar beet, and sesame processing byproducts. Int. J. Food Prop. 2013;16:1148–1168. doi: 10.1080/10942912.2011.578318. [DOI] [Google Scholar]
- 18.Alvares TS, Conte CA, Paschoalin VM, Silva JT, Meirelles CM, Bhambhani YN, Gomes PS. Acute L-arginine supplementation increases muscle blood volume but not strength performance. Appl. Physiol. Nutr. Me. 2012;37:115–126. doi: 10.1139/h11-144. [DOI] [PubMed] [Google Scholar]
- 19.Hernández JL, González-Castro MJ, Alba NI, García CC. High-performance liquid chromatographic determination of mono-and oligosaccharides in vegetables with evaporative light-scattering detection and refractive index detection. J. Chromatogr. Sci. 1998;36:293–298. doi: 10.1093/chromsci/36.6.293. [DOI] [Google Scholar]
- 20.Bavec M, Turinek M, Grobelnik-MLakar S, Slatnar A, Bavec F. Influence of industrial and alternative farming systems on contents of sugars, organic acids, total phenolic content, and the antioxidant activity of red beet (Beta vulgaris L. ssp. vulgaris Rote Kugel) J. Agr. Food Chem. 2010;58:11825–11831. doi: 10.1021/jf103085p. [DOI] [PubMed] [Google Scholar]
- 21.Sun Q, Han Z, Wang L, Xiong L. Physicochemical differences between sorghum starch and sorghum flour modified by heat-moisture treatment. Food Chem. 2014;145:756–764. doi: 10.1016/j.foodchem.2013.08.129. [DOI] [PubMed] [Google Scholar]
- 22.Ratti C. Hot air and freeze-drying of high-value foods: A review. J. Food Eng. 2001;49:311–319. doi: 10.1016/S0260-8774(00)00228-4. [DOI] [Google Scholar]
- 23.Ravichandran K, Saw NMMT, Mohdaly AAA, Gabr MMA, Kastell A, Riedel H, Cai Z, Knorr D, Smetanska I. Impact of processing of red beet on betalain content and antioxidant activity. Food Res. Int. 2013;50:670–675. doi: 10.1016/j.foodres.2011.07.002. [DOI] [Google Scholar]
- 24.Attoe EL, von Elbe JH. Degradation kinetics of betanine in solutions as influenced by oxygen. J. Agr. Food Chem. 1982;30:708–712. doi: 10.1021/jf00112a021. [DOI] [Google Scholar]
- 25.Herbach KM, Stintzing FC, Carle R. Betalain stability and degradation-Structural and chromatic aspects. J. Sci. Food Agr. 2006;71:R41–R50. doi: 10.1111/j.1750-3841.2006.00022.x. [DOI] [Google Scholar]
- 26.Figiel A. Drying kinetics and quality of beetroots dehydrated by combination of convective and vacuum-microwave methods. J. Food Eng. 2010;98:461–470. doi: 10.1016/j.jfoodeng.2010.01.029. [DOI] [Google Scholar]
- 27.Jiménez-Monreal AM, García-Diz L, Martínez-Tomé M, Mariscal M, Murcia MA. Influence of cooking methods on antioxidant activity of vegetables. J. Food Sci. 2009;74:H97–H103. doi: 10.1111/j.1750-3841.2009.01091.x. [DOI] [PubMed] [Google Scholar]
- 28.Chun OK, Kim D-O, Smith N, Schroeder D, Han JT, Lee CY. Daily consumption of phenolics and total antioxidant capacity from fruit and vegetables in the American diet. J. Sci. Food Agr. 2005;85:1715–1724. doi: 10.1002/jsfa.2176. [DOI] [Google Scholar]
- 29.Raczuk J, Wadas W, Glozak K. Nitrates and nitrites in selected vegetables purchased at supermarkets in Siedlce, Poland. Rocz. Panstw. Zakl. Hig. 2014;65:15–20. [PubMed] [Google Scholar]
- 30.Tsikas D, Fuchs I, Gutzki FM, Frölich JC. Measurement of nitrite and nitrate in plasma, serum and urine of humans by high-performance liquid chromatography, the Griess assay, chemiluminescence and gas chromatography-mass spectrometry: Interferences by biogenic amines and N(G)-nitro-L-arginine analogs. J. Chromatogr. 1998;715:441–444. doi: 10.1016/S0378-4347(98)00326-0. [DOI] [PubMed] [Google Scholar]
- 31.Clements WT, Lee SR, Bloomer RJ. Nitrate ingestion: A review of the health and physical performance effects. Nutrient. 2014;6:5224–5264. doi: 10.3390/nu6115224. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 32.Bach V, Mikkelsen L, Kidmose U, Edelenbos M. Culinary preparation of beetroot (Beta vulgaris L.): The impact on sensory quality and appropriateness. J. Sci. Food Agr. 2015;95:1852–1859. doi: 10.1002/jsfa.6886. [DOI] [PubMed] [Google Scholar]
- 33.Rodríguez-Sevilla MD, Villanueva-Suárez MJ, Redondo-Cuenca A. Effects of processing conditions on soluble sugars content of carrot, beetroot and turnip. Food Chem. 1999;66:81–85. doi: 10.1016/S0308-8146(98)00243-X. [DOI] [Google Scholar]
- 34.American Dietetic Association; Dietitians of Canada; American College of Sports Medicine, Rodriguez NR, Di Marco NM, Langley S. American College of Sports Medicine position stand. Nutrition and athletic performance. Med. Sci. Sport. Exer. 41: 709–731 (2009) [DOI] [PubMed]
- 35.Santos PHS, Silva MA. Retention of vitamin c in drying processes of fruits and vegetables-A review. Dry Technol. 2008;26:1421–1437. doi: 10.1080/07373930802458911. [DOI] [Google Scholar]
- 36.Pierucci AP, Andrade LR, Baptista EB, Volpato NM, Rocha-Leão MH. New microencapsulation system for ascorbic acid using pea protein concentrate as coat protector. J. Microencapsul. 2006;23:654–662. doi: 10.1080/02652040600776523. [DOI] [PubMed] [Google Scholar]
- 37.Jiratanan T, Liu RH. Antioxidant activity of processed table beets (Beta vulgaris var, conditiva) and green beans (Phaseolus vulgaris L.) J. Agr. Food Chem. 2004;52:2659–2670. doi: 10.1021/jf034861d. [DOI] [PubMed] [Google Scholar]
- 38.Institute of Medicine (US) Subcommittee on Interpretation and Uses of Dietary Reference Intakes, Institute of Medicine (US) Standing Committee on the Scientific Evaluation of Dietary Reference Intakes. DRI Dietary Reference Intakes: Applications in Dietary Assessment. Washington (DC): National Academies Press (US) (2000) [PubMed]