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. 2016 Mar 31;25(Suppl 1):91–96. doi: 10.1007/s10068-016-0103-x

Partial purification and characterization of polyphenol oxidase from Chinese parsley (Coriandrum sativum)

Hanbin Lin 1, Allan Wee Ren Ng 1, Chen Wai Wong 1,✉
PMCID: PMC6049417  PMID: 30263491

Abstract

Purification and characterization of polyphenol oxidase (PPO) from Chinese parsley (Coriandrum sativum) were achieved. Crude PPO exhibited an enzyme activity of 1,952.24 EU/mL. PPO was partially purified up to 6.52x with a 10.89% yield using gel filtration chromatography. Maximal PPO activity was found at 35°C, pH 8.0 for 4-methylcatechol and at 40°C, pH 7.0 for catechol. PPO showed a higher affinity towards 4-methylcatechol, but a higher thermal stability when reacting with catechol. LCysteine was a better inhibitor than citric acid for reducing PPO activity at concentrations of 1 and 3mM in the presence of either substrate. Two 46 kDa isoenzymes were identified using SDS-PAGE. Isolation and characterization of Chinese parsley serves as a guideline for prediction of enzyme behavior leading to effective prevention of enzymatic browning during processing and storage, including inhibition and inactivation of PPO.

Keywords: polyphenol oxidase, Chinese parsley, characterization

References

  • 1.Mathias ME. Magic, myth and medicine. Econ. Bot. 1994;48:3–7. doi: 10.1007/BF02901373. [DOI] [Google Scholar]
  • 2.Dapkevicius A, Venskutonis R, van Beek TA, Linssen JPH. Antioxidant activity of extracts obtained by different isolation procedures from some aromatic herbs grown in Lithuania. J. Sci. Food Agr. 1998;77:140–146. doi: 10.1002/(SICI)1097-0010(199805)77:1<140::AID-JSFA18>3.0.CO;2-K. [DOI] [Google Scholar]
  • 3.Park G, Kim HG, Kim YO, Park SH, Kim SY, Oh MS. Coriandrum sativum L. protects human keratinocytes from oxidative stress by regulating oxidative defense systems. Skin Pharmacol. Physiol. 2012;25:93–99. doi: 10.1159/000335257. [DOI] [PubMed] [Google Scholar]
  • 4.Rajeshwari U, Andallu B. Medicinal benefits of coriander (Coriandrum sativum L.) Spatula DD. 2011;1:51–58. doi: 10.5455/spatula.20110106123153. [DOI] [Google Scholar]
  • 5.Yoruk R, Marshall MR. Physiochemical properties and functions of plant polyphenol oxidase: A review. J. Food Biochem. 2003;27:361–422. doi: 10.1111/j.1745-4514.2003.tb00289.x. [DOI] [Google Scholar]
  • 6.Mathew AG, Parpia HAB. Food browning as a polyphenol reaction. In: Chichester CO, Mrak EM, Stewart GF, editors. Advances in Food Research. 1971. pp. 75–132. [Google Scholar]
  • 7.Sapers GM, Hicks KB. Inhibition of enzymatic browning in fruits and vegetables. In: Jen JJ, editor. Quality Factors of Fruits and Vegetables. 1989. pp. 29–43. [Google Scholar]
  • 8.Whitaker JR, Lee CY. Recent advances in chemistry of enzymatic browning. In: Lee CY, Whitaker JR, editors. Enzymatic Browning and Its Prevention. 1995. pp. 2–7. [Google Scholar]
  • 9.Queiroz C, Mendes Lopes ML, Fialho E, Valente-Mesquita VL. Polyphenol oxidase: Characteristics and mechanisms of browning control. Food Rev. Int. 2008;24:361–375. doi: 10.1080/87559120802089332. [DOI] [Google Scholar]
  • 10.Nuñez-Delicado E, Serrano-Megías M, Pérez-López AJ, López-Nicolás JM. Polyphenol oxidase from Dominga table grape. J. Agr. Food Chem. 2005;53:6087–6093. doi: 10.1021/jf050346z. [DOI] [PubMed] [Google Scholar]
  • 11.Ng AWR, Wong CW. Partial purification and characterization of polyphenol oxidase from round brinjal (Solanum melongena var. depressum) Int. Food Res. J. 2015;22:833–838. [Google Scholar]
  • 12.Kumar VBA, Mohan TCK, Murugan K. Purification and kinetic characterization of polyphenol oxidase from Barbados cherry (Malpighia glabra L.) Food Chem. 2008;110:328–333. doi: 10.1016/j.foodchem.2008.02.006. [DOI] [PubMed] [Google Scholar]
  • 13.Wong Y, Yu J. Laccase-catalyzed decolorization of synthetic dyes. Water Res. 1999;33:3512–3520. doi: 10.1016/S0043-1354(99)00066-4. [DOI] [Google Scholar]
  • 14.Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 1951;193:265–275. [PubMed] [Google Scholar]
  • 15.Eidhin DN, Degn P, O’beirne D. Characterization of polyphenol oxidase from rooster potato (Solanum tuberosum CV Rooster) J. Food Biochem. 2010;34:13–30. doi: 10.1111/j.1745-4514.2009.00256.x. [DOI] [Google Scholar]
  • 16.Ziyan E, Pekyardimci S. Purification and characterization of pear (Pyrus communis) polyphenol oxidase. Turk. J. Chem. 2004;28:547–557. [Google Scholar]
  • 17.Dogan S, Turan Y, Ertruk H, Arslan O. Characterization and purification of polyphenol oxidase from artichoke (Cynaras colymus L.) J. Agr. Food Chem. 2005;53:776–785. doi: 10.1021/jf049053g. [DOI] [PubMed] [Google Scholar]
  • 18.Gundoggmaz G, Doggan S, Arslan O. Some kinetic properties of polyphenol oxidase obtained from various Salvia species (Salvia viridis L., Salvia virgate Jacq. and Salvia tomentosa Miller) Food Sci. Technol. Int. 2003;9:309–315. doi: 10.1177/108201303036476. [DOI] [Google Scholar]
  • 19.Dogru YZ, Erat M. Investigation of some kinetic properties of polyphenol oxidase from parsley (Petroselinum crispum, Apiaceae) Food Res. Int. 2012;49:411–415. doi: 10.1016/j.foodres.2012.07.028. [DOI] [Google Scholar]
  • 20.Dogru YZ, Erat M. Investigation of some kinetic properties of polyphenol oxidase from parsley (Petroselinum crispum, Apiaceae) Food Res. Int. 2012;49:411–415. doi: 10.1016/j.foodres.2012.07.028. [DOI] [Google Scholar]
  • 21.Cho YK, Ahn HK. Purification and characterization of polyphenol oxidase from potato: I. Purification and properties. J. Food Biochem. 1999;23:577–592. doi: 10.1111/j.1745-4514.1999.tb00587.x. [DOI] [Google Scholar]
  • 22.Gauillard F, Richard-Forget F. Polyphenoloxidases from Williams pear (Pyrus communis L., cv Williams): Activation, purification and some properties. J. Sci. Food Agr. 1997;74:49–56. doi: 10.1002/(SICI)1097-0010(199705)74:1<49::AID-JSFA769>3.0.CO;2-K. [DOI] [Google Scholar]
  • 23.Zhou P, Smith NL, Lee CY. Potential purification and some properties of Monroe apple peel polyphenol oxidase. J. Agr. Food Chem. 1993;41:532–536. doi: 10.1021/jf00028a004. [DOI] [Google Scholar]
  • 24.Yang CP, Fujita S, Ashrafuzzaman MD, Nakamura N, Hayashi N. Purification and characterization of polyphenol oxidase from banana (Musa sapientum L.) pulp. J. Agr. Food Chem. 2000;48:2732–2735. doi: 10.1021/jf991037+. [DOI] [PubMed] [Google Scholar]
  • 25.Dincer B, Colak A, Aydin N, Kadioglu A, Güner S. Characterization of polyphenol oxidase from medlar fruits (Mespilus germanica L., Rosaceae) Food Chem. 2002;77:1–7. doi: 10.1016/S0308-8146(01)00359-4. [DOI] [Google Scholar]
  • 26.Nagai T, Suzuki N. Partial purification of polyphenol oxidase from Chinese cabbage (Brassica rapa L.) J. Agr. Food Chem. 2001;49:3922–3926. doi: 10.1021/jf000694v. [DOI] [PubMed] [Google Scholar]
  • 27.Oktay M, Küfreviolu I, Kocaçaliskan I, Sakirolu H. Polyphenol oxidase from Amasya apple. J. Food Sci. 1995;60:494–496. doi: 10.1111/j.1365-2621.1995.tb09810.x. [DOI] [Google Scholar]
  • 28.Wesche-Ebleing P, Montgomery MW. Strawberry polyphenol oxidase: Extraction and partial characterization. J. Food Sci. 1990;55:1320–1325. doi: 10.1111/j.1365-2621.1990.tb03925.x. [DOI] [Google Scholar]
  • 29.Zhou HW, Feng X. Polyphenol oxidase from Yali pear (Pyrus bretschneideri) J. Sci. Food Agr. 1991;57:307–313. doi: 10.1002/jsfa.2740570302. [DOI] [Google Scholar]
  • 30.Altunkaya A, Gökmen V. Partial purification and characterization of polyphenoloxidase from durum wheat (Triticum durum L.) J. Cereal Sci. 2012;55:300–304. doi: 10.1016/j.jcs.2011.12.013. [DOI] [Google Scholar]
  • 31.Duangmal K. Apenten RKO. A comparative study of polyphenol oxidase from taro (Colocasia esculenta) and potato (Solanum tuberosum var. Romano) Food Chem. 1999;64:351–359. doi: 10.1016/S0308-8146(98)00127-7. [DOI] [Google Scholar]
  • 32.Katwa LC, Ramakrishna M, Rao MRR. Purification and properties of polyphenol oxidase from mango peel (Mangifera indica var. Raspuri) J. Food Biochem. 1982;6:217–228. doi: 10.1111/j.1745-4514.1982.tb00303.x. [DOI] [Google Scholar]
  • 33.Kahn V. Some biochemical properties of polyphenol oxidase from two avocado varieties differing in their browning rates. J. Food Sci. 1977;42:38–43. doi: 10.1111/j.1365-2621.1977.tb01213.x. [DOI] [Google Scholar]
  • 34.Iyidogan NF, Bayindirli A. Effect of L-cysteine, kojic acid and 4-hexylresorcinal combination on inhibition of enzymatic browning in Amasya apple juice. J. Food Eng. 2004;62:299–304. doi: 10.1016/S0260-8774(03)00243-7. [DOI] [Google Scholar]
  • 35.Gacche RN, Shete AM, Dhole NA, Ghole VS. Reversible inhibition of polyphenol oxidase from apple using l-cysteine. Indian J. Chem. Techn. 2006;13:459–463. [Google Scholar]

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