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. 2016 Mar 31;25(Suppl 1):15–21. doi: 10.1007/s10068-016-0093-8

Impact of stirring speed on β-lactoglobulin fibril formation

Shy Kai Ng 1,✉, Kar Lin Nyam 2, Imededdine Arbi Nehdi 3, Gun Hean Chong 1, Oi Ming Lai 4, Chin Ping Tan 1
PMCID: PMC6049419  PMID: 30263481

Abstract

β-Lactoglobulin (β-lg) can produce fibrils that have multi-functional properties. Impacts of different stirring speeds on characteristics of β-lg fibrils as a stable form in β-lg fibril solutions were investigated. Fibril concentration, fibril morphology, turbidity, particle size distribution, zeta potential, and rheological behavior of solutions were studied. Stirring enhanced fibril formation and stability of a fibril solution, in comparison with unstirred solutions. Increasing the stirring speed produced more turbidity and a greater distribution of particle sizes, higher viscosity values, but no differences in zeta potential values of β-lg fibril solutions. However, a high stirring speed is not feasible due to reduction of the fibril yield and changes in fibril morphology.

Keywords: fibril, β-lactoglobulin, stirring, zeta potential, morphology

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