Abstract
This study was conducted to compare the organic acid content in liquors (red wine, white wine, and beer) using three different high-performance liquid chromatography analysis methods. Post-column reaction methods (method 2 and 3) were found to be more promising than UV-detection method (method 1). Using method 2 (two columns), the analyzed red wine was found to contain 2,652.4 mg/L tartaric acid and 1,392.9 mg/L lactic acid but relatively lower amounts of malic acid (271.0 mg/L). Furthermore, tartaric acid (1,160.8–2,749.1 mg/L) and malic acid (470.2–3,107.9 mg/L) were the major components in white wine. Beers were analyzed using method 3 (one column). In the analyzed foreign beers, lactic acid (95.9–226.4 mg/L), malic acid (62.2–110.5 mg/L), acetic acid (93.5–183.8 mg/L), and succinic acid (37.0–56.2 mg/L) were detected. Similar to the foreign beers, the contents of succinic acid in the domestic beers were the lowest. The proposed methods could be useful for quantitative analysis of organic acids in wine and beer.
Keywords: organic acids, UV-detection, post-column reaction, wine, beer
References
- 1.Whiting GC. Organic acid metabolism of yeasts during fermentation of alcoholic beverages—A review. J. I. Brewing. 1976;82:84–92. doi: 10.1002/j.2050-0416.1976.tb03731.x. [DOI] [Google Scholar]
- 2.Davis CR, Wibowo D, Eschenbruch R, Lee TH, Fleet GH. Practical implications of malolactic fermentation: A review. Am. J. Enol. Viticult. 1985;36:290–301. [Google Scholar]
- 3.Versari A, Parpinello GP, Cattaneo M. Leuconostoc oenos and malolactic fermentation in wine: A review. J. Ind. Microbiol. Biot. 1999;23:447–455. doi: 10.1038/sj.jim.2900733. [DOI] [Google Scholar]
- 4.Henick-Kling T. Control of malo-lactic fermentation in wine: Energetics, flavour modification and methods of starter culture preparation. J. Appl. Bacteriol. Symp. Suppl. 1995;79:29S–37S. [Google Scholar]
- 5.Nour V, Trandafir I, Ionica ME. HPLC organic acid analysis in different citrus juices under reversed phase conditions. Not. Bot. Horti Agrobo. 2010;38:44–48. [Google Scholar]
- 6.Chen QY, Xiao JB, Chen XQ. Rapid determination of organic acids in Bayer liquors by high-performance liquid chromatography after solid-phase extraction. Miner. Eng. 2006;19:1446–1451. doi: 10.1016/j.mineng.2006.03.015. [DOI] [Google Scholar]
- 7.Peres RG, Moraes EP, Micke GA, Tonin FG, Tavares MFM, Rodriguez-Amaya DB. Rapid method for the determination of organic acids in wine by capillary electrophoresis with indirect UV detection. Food Control. 2009;20:548–552. doi: 10.1016/j.foodcont.2008.08.004. [DOI] [Google Scholar]
- 8.Regmi U, Palma M, Barroso CG. Direct determination of organic acids in wine and wine-derived products by Fourier transform infrared (FT-IR) spectroscopy and chemometric techniques. Anal. Chim. Acta. 2012;732:137–144. doi: 10.1016/j.aca.2011.11.009. [DOI] [PubMed] [Google Scholar]
- 9.Käkölä JM, Alén RJ, Isoaho JP, Matilainen RB. Determination of low-molecularmass aliphatic carboxylic acids and inorganic anions from kraft black liquors by ion chromatography. J. Chromatogr. A. 2008;1190:150–156. doi: 10.1016/j.chroma.2008.02.096. [DOI] [PubMed] [Google Scholar]
- 10.Nord LI, Vaag P, Duus J. Quantification of organic and amino acids in beer by 1H NMR spectroscopy. Anal. Chem. 2004;76:4790–4798. doi: 10.1021/ac0496852. [DOI] [PubMed] [Google Scholar]
- 11.The International Organization of Vine and Wine (OIV). Method for the determination of organic acid compounds of wine by HPLC (OIV-MA-AS313-04) (2010)
- 12.Shodex. Application guide of organic acids in beer. Available from: http://www.shodex.com. Accessed Oct. 20, 2016.
- 13.Tabaru H, Kadota E, Yamada H, Sasaki N, Takeuchi A. Determination of volatile fatty acids and lactic acid in bovine plasma and ruminal fluid by high performance liquid chromatography. Jpn. J. Vet. Sci. 1988;50:1124–1126. doi: 10.1292/jvms1939.50.1124. [DOI] [PubMed] [Google Scholar]
- 14.Castineira A, Pena RM, Herrero C, Garcia-Martin S. Analysis of organic acids in wine by capillary electrophoresis with direct UV detection. J. Food Compos. Anal. 2002;15:319–331. doi: 10.1006/jfca.2002.1056. [DOI] [Google Scholar]
- 15.Montanari L, Perretti G, Natella F, Guidi A, Fantozzi P. Organic and phenolic acids in beer. LWT-Food Sci. Technol. 1999;32:535–539. doi: 10.1006/fstl.1999.0593. [DOI] [Google Scholar]