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. 2017 Apr 30;26(2):481–487. doi: 10.1007/s10068-017-0066-6

Inhibitory effect of Zizania latifolia chloroform fraction on allergy-related mediator production in RBL-2H3 cells

Eun-Jung Lee 1, Mi-Hee Yu 1, Coralia V Garcia 1, Kwang-Hwan Jhee 2, Seun-Ah Yang 1,✉
PMCID: PMC6049454  PMID: 30263568

Abstract

Zizania latifolia exhibits anti-inflammatory and anti-allergic effects; however, the mechanisms behind these effects are unknown. Here the ethanol extract of Z. latifolia was partitioned using hexane, chloroform, ethyl acetate, butanol, and water. Subsequently, the anti-allergic effects of these fractions were evaluated in vitro. The results showed that the chloroform fraction of Z. latifolia inhibited the release of β-hexosaminidase and tumor necrosis factor (TNF-α) from RBL-2H3 cells stimulated with dinitrophenyl-bovine serum albumin (DNP-BSA). In addition, this fraction suppressed the expression of cyclooxygenase-2 (COX-2) and inhibited the activation of mitogen-activated protein kinases (MAPKs). The results obtained suggest that the chloroform fraction of Z. latifolia inhibited mast cell-mediated allergic inflammatory responses.

Keywords: Zizania latifolia, anti-allergic, RBL-2H3 cells, COX-2, MAPK

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