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. 2017 Feb 28;26(1):287–291. doi: 10.1007/s10068-017-0038-x

Antimicrobial susceptibility and serotyping of Listeria monocytogenes isolated from ready-to-eat seafood and food processing environments in Korea

Da Yeon Lee 1, Jae Ho Ha 2, Myung Ki Lee 3, Yong Sun Cho 1,✉
PMCID: PMC6049479  PMID: 30263540

Abstract

We examined antimicrobial susceptibility and serotypes of 33 L. monocytogenes isolates collected from ready-to-eat seafood and food processing environments. The isolated strains belonged to the 1/2b (73%), 4b (15%), and 1/2a (12%) serotypes; 11 of the obtained environmental swab samples belonged to the 1/2b serogroup. Antimicrobial resistance to benzyl penicillin (100%), clindamycin (100%), oxacillin (100%), ampicillin (97%), and tetracycline (18%) was detected, and 27/33 isolates (82%) showed resistance to four antibiotics and 6/33 (18%) were resistant to five. Total typing by automated repetitive sequence-based PCR revealed that the 33 isolates grouped into four distinct clusters with significantly correlated serotypes. These findings provide important information about the safety of ready-to-eat seafood and suggest that control measures should be adopted in order to mitigate the risk to humans posed by L. monocytogenes contaminated seafood.

Keywords: Listeria monocytogenes, seafoods, characterization, antimicrobial susceptibility, rep-PCR

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