Proteins can be conjugated covalently by ubiquitin or ubiquitin-like proteins, such as SUMO and ISG15. ISG15 is highly induced in viral infection, and ISG15 conjugation, termed ISGylation, plays important regulatory roles in viral growth. Although ISGylation has been shown to negatively affect many viruses, including human cytomegalovirus (HCMV), viral countermeasures that might modulate ISGylation are not well understood. In the present study, we show that the transmembrane protein encoded by HCMV UL50 inhibits ISGylation by causing proteasomal degradation of UBE1L, an E1-activating enzyme for ISGylation. This pUL50 activity requires membrane targeting. In support of this finding, RNF170, an ER-associated ubiquitin E3 ligase, interacts with pUL50 and promotes UL50-mediated UBE1L ubiquitination and degradation. Our results provide the first evidence, to our knowledge, that viruses can regulate ISGylation by directly targeting the ISGylation E1 enzyme.
KEYWORDS: cytomegalovirus, UL50, ISGylation, UBE1L
ABSTRACT
Interferon-stimulated gene 15 (ISG15) encodes a ubiquitin-like protein that can be conjugated to proteins via an enzymatic cascade involving the E1, E2, and E3 enzymes. ISG15 expression and protein ISGylation modulate viral infection; however, the viral mechanisms regulating the function of ISG15 and ISGylation are not well understood. We recently showed that ISGylation suppresses the growth of human cytomegalovirus (HCMV) at multiple steps of the virus life cycle and that the virus-encoded pUL26 protein inhibits protein ISGylation. In this study, we demonstrate that the HCMV UL50-encoded transmembrane protein, a component of the nuclear egress complex, also inhibits ISGylation. pUL50 interacted with UBE1L, an E1-activating enzyme for ISGylation, and (to a lesser extent) with ISG15, as did pUL26. However, unlike pUL26, pUL50 caused proteasomal degradation of UBE1L. The UBE1L level induced in human fibroblast cells by interferon beta treatment or virus infection was reduced by pUL50 expression. This activity of pUL50 involved the transmembrane (TM) domain within its C-terminal region, although pUL50 could interact with UBE1L in a manner independent of the TM domain. Consistently, colocalization of pUL50 with UBE1L was observed in cells treated with a proteasome inhibitor. Furthermore, we found that RNF170, an endoplasmic reticulum (ER)-associated ubiquitin E3 ligase, interacted with pUL50 and promoted pUL50-mediated UBE1L degradation via ubiquitination. Our results demonstrate a novel role for the pUL50 transmembrane protein of HCMV in the regulation of protein ISGylation.
IMPORTANCE Proteins can be conjugated covalently by ubiquitin or ubiquitin-like proteins, such as SUMO and ISG15. ISG15 is highly induced in viral infection, and ISG15 conjugation, termed ISGylation, plays important regulatory roles in viral growth. Although ISGylation has been shown to negatively affect many viruses, including human cytomegalovirus (HCMV), viral countermeasures that might modulate ISGylation are not well understood. In the present study, we show that the transmembrane protein encoded by HCMV UL50 inhibits ISGylation by causing proteasomal degradation of UBE1L, an E1-activating enzyme for ISGylation. This pUL50 activity requires membrane targeting. In support of this finding, RNF170, an ER-associated ubiquitin E3 ligase, interacts with pUL50 and promotes UL50-mediated UBE1L ubiquitination and degradation. Our results provide the first evidence, to our knowledge, that viruses can regulate ISGylation by directly targeting the ISGylation E1 enzyme.
INTRODUCTION
Interferon-stimulated gene 15 (ISG15) encodes a ubiquitin-like protein that is covalently conjugated to lysine residues of a substrate protein through an enzymatic cascade involving E1, E2, and E3 enzymes, similar to the ubiquitin conjugation pathway (1, 2). UBE1L is the E1-activating enzyme for ISG15, and UbcH8, a ubiquitin E2-conjugating enzyme, also functions as the ISG15 E2-conjugating enzyme. E3 ligases for ISGylation in human cells include the HERC domain- and RCC1-like domain-containing protein 5 (Herc5), estrogen-responsive finger protein (EFP), and a human homolog of the Drosophila ariadne gene product (HHARI). ISG15, UBE1L, UbcH8, and Herc5 are all interferon (IFN) inducible. ISG15 can be removed reversibly from substrates by an ISG15-specific protease, USP18 (also known as UBP43), which is also IFN inducible (reviewed in reference 3). USP18 also acts as a negative regulator of the innate immune response, independent of its ISG15-deconjugating (deISGylating) activity, by association with a subunit of the type I IFN receptor, IFNAR2 (4, 5).
ISG15 or ISGylation represses the growth of diverse viruses, including influenza virus (types A and B), human immunodeficiency virus, avian sarcoma/leukosis virus, hepatitis C virus, Japanese encephalitis virus, Sindbis virus, vesicular stomatitis virus, dengue virus, West Nile virus, Ebola virus, porcine reproductive and respiratory syndrome virus, Chikungunya virus, herpes simplex virus 1 (HSV-1), murine gammaherpesvirus 68, and vaccinia virus (VACV) (6, 7; reviewed in reference 8). Recently, the antiviral activities of ISG15 or ISGylation were also demonstrated for Kaposi's sarcoma-associated herpesvirus (KSHV) (9), respiratory syncytial virus (10), and human cytomegalovirus (HCMV) (11, 12). In contrast to the antiviral activities of ISG15, free ISG15 has also been shown to negatively regulate the type I IFN response in humans by promoting sustained expression of USP18, which negatively regulates IFN signaling, suggesting an ISGylation-independent role for ISG15 in IFN pathway regulation (13–16).
Studies on the antiviral mechanisms of ISG15 have demonstrated that ISGylation regulates various steps of the virus life cycle. ISGylation can directly inhibit the functions of viral proteins. ISGylation of the influenza A virus (IAV) NS1 protein inhibits its binding to importin-α, blocking NS1 nuclear import (17), while ISGylation of the influenza B virus (IBV) NP protein inhibits its oligomerization, inhibiting the formation of the viral ribonucleoprotein complex (18). ISGylation of HCMV pUL26 regulates its ubiquitination and inhibits its activity to suppress tumor necrosis factor alpha (TNF-α)-mediated NF-κB activation (12). ISGylation can also affect viral growth by regulating cellular proteins. ISGylation of Nedd4 inhibits release of Ebola virus VP40 virus-like particles (19), while ISGylation of CHMP5 inhibits release of retroviruses (20, 21), and probably HCMV (12). ISGylation also affects microRNA (miRNA) functions (22) and the formation of autophagic clusters, inhibiting the growth of KSHV and HSV-1, respectively (23). Furthermore, ISGylation affects the early steps of the virus life cycle, such as virus entry of norovirus and viral gene expression in HCMV (12, 24).
Viral countermeasures against the antiviral effects of ISG15 and/or ISGylation have been demonstrated for some viruses. The IBV NS1 protein binds to ISG15 and suppresses the levels of ISGylation as it prevents the E1 enzyme from conjugating ISG15 (25). Furthermore, binding of NS1 to ISGylated NP protein appears to block the dominant negative effect of ISGylated NP on viral RNA synthesis (18). In VACV infection, the VACV E3 protein can bind to ISG15 and inhibit its antiviral activity (26). During HCMV infection, pUL26 binds to ISG15, UBE1L, and Herc5, and these interactions appear to be responsible for inhibiting ISGylation (12). Viruses also encode deISGylation enzymes. Nairoviruses and arteriviruses encode an ovarian tumor (OTU) domain-containing protease (27), while severe acute respiratory syndrome coronavirus (SARS-CoV) encodes papain-like protease (PLpro) enzymes that cleave ISG15 from its target proteins (28). However, viral mechanisms that regulate ISGylation enzymes are less well known.
HCMV belongs to the betaherpesvirus subfamily and typically causes latent and persistent infection. However, reactivation of virus in immunocompromised individuals, such as AIDS patients and transplantation recipients, and primary infections of newborns can result in severe disease (29). During lytic infection, HCMV gene expression occurs in a three-step sequential fashion, with immediate early (IE), early (E), and late (L) gene expression. HCMV expresses proteins that are able to counteract IFN production and subsequent ISG activation. IE2 and pp65 inhibit IFN production (30–32), whereas IE1 directly interacts with STAT2 and PML to suppress the IFN response (33–37). HCMV infection reduces levels of JAK1 and p48, two components of the type I IFN signaling pathway (38, 39). The stability and phosphorylation of STAT proteins are modulated during HCMV infection (40, 41). ISG15 expression and levels of ISGylation are upregulated immediately after HCMV infection via signaling pathways, including one mediated by cGAS/STING (11). However, HCMV appears to be armed with countermeasures, such as pUL26, which inhibits ISGylation at later stages of infection (12).
During HCMV infection, the core of the viral nuclear egress complex (NEC) is formed by two conserved viral proteins: pUL50 and pUL53. The integral membrane protein pUL50 is associated with the inner nuclear membrane (INM) by its transmembrane (TM) domain, whereas the nuclear phosphoprotein pUL53 carries a classical nuclear localization signal and accumulates in the nuclear rim upon interaction with the INM-associated pUL50 protein. Once the core NEC is formed, further NEC proteins, such as p32/gC1qR, emerin, and protein kinases, such as the viral protein kinase pUL97, cellular protein kinase C isoform a (PKCa), and cellular cyclin-dependent kinase 1 (CDK1), are recruited (42–46) in order to phosphorylate nuclear lamins, resulting in lamina disassembly (47–49). The recently solved crystal structure of the pUL50-pUL53 complex suggests that pUL50-pUL53 heterodimers assemble to form hexameric ring-like structures, acting as a scaffold for binding of further NEC proteins (50). The association of nuclear capsids with the NEC proteins has been demonstrated (51).
In this study, we demonstrate that the HCMV-encoded transmembrane protein pUL50 acts as a negative regulator of protein ISGylation by interacting with UBE1L and inducing its proteasomal degradation. We also show that this activity of pUL50 requires its transmembrane domain. Furthermore, we demonstrate that RNF170, an endoplasmic reticulum (ER)-associated ubiquitin E3 ligase, interacts with pUL50 and is involved in pUL50-mediated UBE1L degradation.
RESULTS
HCMV pUL50 interacts with UBE1L.
To identify HCMV proteins that regulate the ISG15 pathway, we performed yeast two-hybrid interaction assays and identified the transmembrane protein pUL50, encoded by HCMV UL50, as a potential UBE1L-binding protein. We further investigated the interaction of pUL50 with components of the ISG15 pathway. When we performed coimmunoprecipitation (co-IP) assays by using 293T cells cotransfected with UL50 and UBE1L, UbcH8, Herc5, or ISG15AA (a conjugation-inactive form), pUL50 interacted strongly with UBE1L and to a lesser extent with ISG15AA, while showing weak or no binding to UbcH8 and Herc5 (Fig. 1A). An isoform of pUL50 of about 26 kDa (denoted UL50-p26), which appears to start from an internal methionine, was also expressed from the UL50 gene. To further investigate the interaction of pUL50 with UBE1L and ISG15 during HCMV infection, we used a recombinant HCMV strain that expresses pUL50 with a C-terminal hemagglutinin (HA) tag. Although this recombinant virus showed reduced growth compared to that of its parental virus, which might be due to limited stability of the tagged protein (52), the pUL50-HA protein expressed from the recombinant virus was localized normally at the nuclear rim and interacted with all known protein ligands (53). In HF cells infected with this UL50-HA virus, immunoprecipitation of pUL50-HA with anti-HA antibody coprecipitated UBE1L and ISG15, while immunoprecipitation with anti-Flag antibody (as a control) did not (Fig. 1B and C). The results of these co-IP assays confirmed the specific interaction of pUL50 with UBE1L and ISG15 in both transfected and virus-infected cells.
pUL50 inhibits ISGylation and causes proteasomal degradation of UBE1L.
We investigated whether pUL50, like HCMV pUL26 (12), regulates protein ISGylation by using cotransfection/ISGylation assays, in which ISGylated proteins are detected in cells cotransfected with plasmids encoding ISG15GG (an active form), UBE1L, UbcH8, and Herc5. We found that pUL50 expression downregulated ISGylation in this assay in a dose-dependent manner (Fig. 2).
We next examined the effect of pUL50 on the expression levels of individual components of the ISG15 pathway by using cotransfection assays. pUL50 expression effectively reduced UBE1L in a dose-dependent manner, although high-level expression of pUL50 also reduced Herc5 and, to a lesser extent, ISG15. Treatment of cells with MG132, a proteasome inhibitor, largely restored the loss of UBE1L, Herc5, and ISG15 (Fig. 3A, B, and D). pUL50 alone did not downregulate UbcH8 expression, although MG132 treatment appeared to affect the UbcH8 level (Fig. 3C). As a specificity control, pUL26, which inhibits ISGylation, did not reduce the level of UBE1L (Fig. 3E). These results demonstrate that pUL50 expression causes degradation of UBE1L and (to a lesser extent) Herc5 and ISG15 through proteasomal activity. Consistently, the reduction of UBE1L expression by pUL50 was not seen at the transcriptional level (Fig. 1F). Since UBE1L appeared to be regulated directly by pUL50 through a relatively strong interaction, we focused on UBE1L regulation by pUL50 expression.
As an IFN-inducible gene, UBE1L was highly induced in HF cells infected with UV-inactivated HCMV (UV-HCMV), but this induction was mitigated with intact HCMV infection due to the expression of viral gene products that counteract IFN-β production and activation of ISGs (Fig. 4A). We investigated whether pUL50 expression affects levels of UBE1L in HF cells induced by IFN-β treatment or virus infection. Control HF cells and cells expressing pUL50-HA were produced by use of retroviral vectors. After induction of UBE1L by IFN-β treatment, cells were treated with cycloheximide (CHX), and the level of UBE1L was monitored. The half-life of IFN-β-induced UBE1L became shorter in pUL50-HA-expressing cells than in control cells (compare the UBE1L levels 4 h after CHX treatment in Fig. 4B, lanes 3 and 8). The half-life of pUL50-HA was shorter than that of UBE1L, and the reduction of pUL50-HA expression from 8 h after CHX treatment restored UBE1L levels (Fig. 4B, lanes 9 and 10). We also found that the expression of pUL50-HA in HF cells by recombinant adenoviral vectors reduced the UBE1L level induced by UV-HCMV infection (Fig. 4C). Collectively, these results show that pUL50 expression downregulates the expression of endogenous UBE1L in HF cells.
pUL50 requires the TM domain in its C-terminal region for ISGylation inhibition.
pUL50 consists of the conserved N-terminal globular domain, including the pUL53-binding region, and a C-terminal region containing the transmembrane (TM) domain (50, 54). To identify the region of pUL50 required for ISGylation inhibition, several N- or C-terminally truncated UL50 mutants were produced and used for cotransfection/ISGylation assays. The results suggested that the UL50 constructs containing the C-terminal region encompassing the TM domain effectively inhibited ISGylation. To address the role of the TM domain in ISGylation inhibition, two TM domain-deleted UL50 mutant proteins, ΔTM and 1-358, were produced and used in cotransfection/ISGylation assays. Unlike intact pUL50, both the ΔTM and 1-358 mutants did not inhibit ISGylation or cause the loss of UBE1 and Herc5 (Fig. 5A and B). Both the ΔTM and 1-358 mutants still interacted with UBE1L in co-IP assays (Fig. 5C). Unlike intact pUL50, both mutants failed to accumulate in the nuclear rim of HF cells as expected, suggesting that pUL50 binds to UBE1L independent of its membrane targeting (Fig. 5D).
The activity of pUL50 in binding to and degrading UBE1L was further investigated by indirect immunofluorescence assay (IFA). Unlike wild-type pUL50, the TM domain-deleted UL50 proteins did not accumulate at the nuclear rim and were largely nuclear (Fig. 6A, panels a and b). UBE1L has been shown to be localized largely in the cytoplasm (55). Consistently, UBE1L was mainly distributed in the cytoplasm of HeLa cells (Fig. 6A, panel c). When UBE1L was coexpressed with wild-type pUL50, the pUL50 localization was not apparently altered by UBE1L expression, and UBE1L did not accumulate at high levels at the nuclear rim where pUL50 concentrated (Fig. 6B, panel a); however, when cotransfected cells were treated with MG132, UBE1L and pUL50 were colocalized largely in the nuclear periphery and within the nucleus (Fig. 6B, panel b). When cells were cotransfected with UBE1L and the ΔTM UL50 mutant, the localization of the ΔTM UL50 protein was not affected by UBE1L; however, when cotransfected cells were treated with MG132, the proteins were only partly colocalized within the nucleus (Fig. 6B, panels c and d). Our finding that UBE1L was effectively colocalized with wild-type pUL50 in the nuclear periphery when proteasomal activity was inhibited supports the idea that pUL50 expression causes UBE1L degradation in a manner dependent on its membrane targeting through the TM domain.
The ubiquitin E3 ligase RNF170 is involved in pUL50-mediated UBE1L degradation.
To understand the mechanism by which pUL50 induces UBE1L degradation, we examined the possible role of several ubiquitin E3 ligases in UBE1L degradation. In a previous proteomics study of the NEC interactome (53), several peptides from ubiquitin E3 ligases, such as RNF170, were found to be associated with the NEC, although their significance was low. We found that expression of RNF170 variant 1 (RNF170v1) and variant 3 (RNF170v3) promoted pUL50-mediated UBE1L degradation in cotransfection assays, although expression of RNF170 proteins alone, without pUL50 coexpression, did not affect the UBE1L level (Fig. 7A and B). In co-IP assays, RNF170 proteins were found to interact with pUL50 (Fig. 7C). RNF170 proteins have been reported to be associated with the ER (56). RNF170 proteins were colocalized with pUL50 at sites including the nuclear rim in cotransfected HeLa cells (Fig. 8A) and in UL50-HA virus-infected HF cells (Fig. 8B), supporting the results of co-IP assays demonstrating that RNF170 is associated with pUL50.
The roles of pUL50 and RNF170 in UBE1L degradation through proteasomal activity were further investigated by performing cotransfection/ubiquitination assays. We found that pUL50 effectively increased UBE1L ubiquitination, supporting the finding that proteasome activity is required for UBE1L degradation by UL50 (Fig. 9, compare lanes 2 and 4). Furthermore, we found that RNF170 promoted the pUL50-mediated ubiquitination of UBE1L (Fig. 9, compare lanes 2 and 3 and lanes 4 and 5). Taken together, our results suggest that RNF170 is involved in pUL50-mediated UBE1L degradation through the ubiquitin-proteasome pathway.
DISCUSSION
In this study, we demonstrated that the transmembrane protein pUL50 of HCMV interacts with UBE1L, an E1-activating enzyme for ISGylation, and causes its proteasomal degradation. pUL50 expression in HCMV-permissive cells reduced the UBE1L level that was induced by IFN-β treatment or UV-HCMV infection. Studies on the mechanism underlying this pUL50 activity revealed that although the C-terminal TM domain is not essential for UBE1L binding, it is required for UBE1L degradation. Moreover, we found that RNF170, an ER-associated ubiquitin E3 ligase, interacts with pUL50 and promotes pUL50-mediated UBE1L ubiquitination for degradation. Taken together, these results give rise to the idea that pUL50 recruits UBE1L to the ER membrane, where RNF170 facilitates UBE1L ubiquitination for proteasomal degradation. Given the role of the ISGylation pathway in host defense against virus infection (8), the findings of the present study reveal an unexpected role for pUL50, a component of the NEC that mediates the nuclear egress of the capsid, in counteracting the host immune response by downregulating protein ISGylation.
pUL50 is the second HCMV protein identified that inhibits ISGylation. We previously showed that pUL26 interacts with ISG15, UBE1L, and Herc5 and inhibits ISGylation, although the underlying mechanism is unclear (12). In the current study, we also demonstrated that pUL50 interacts with ISG15 and UBE1L. Although both pUL26 and pUL50 target ISG15 and UBE1L, they appear to use different mechanisms to inhibit ISGylation, since only pUL50 induced UBE1L degradation. Given that pUL50 is a membrane protein that targets the ER and eventually the INM to act as a regulator of the nuclear egress of the capsid, the requirement of the TM domain of pUL50 for UBE1L degradation suggests that the ER is the site of UBE1L degradation. In support of this model, pUL50 was colocalized with UBE1L at the nuclear periphery, which appeared to include the compressed ER membrane, in the presence of a proteasome inhibitor. Furthermore, an ER-associated ubiquitin E3 ligase was found to bind to pUL50 and promote UL50-mediated UBE1L degradation. Recently, HSV-1 pUL34, a homolog of HCMV pUL50, was shown to remodel the global ER architecture, including ER compression around the nuclear membrane during virus infection, which may lead to the recruitment of nuclear egress regulators to the nuclear membrane (57). Therefore, it is also an intriguing scenario that the pUL50-triggered remodeling of the ER architecture is responsible for the exposure of UBE1L to ER-associated ubiquitin E3 ligases, such as RNF170. This activity of pUL50 may also be related to the high level of pUL50-induced loss of Herc5, which is associated with polyribosomes, and (to a lesser extent) of ISG15, which weakly interacts with pUL50.
Analyses of the expression of pUL50 with a C-terminal HA tag from transfected or recombinant virus-infected cells revealed that a smaller isoform of pUL50, of about 26 kDa (referred to as UL50-p26 in this study), is expressed from the UL50 gene. This isoform appears to initiate from an internal methionine codon and encompasses the C-terminal half of pUL50, including the TM domain, but not the N-terminal globular domain necessary for pUL53 binding to form the NEC. We observed that this isoform was expressed earlier than pUL50 in virus-infected cells, but it became less abundant at the late phases of infection and was capable of moderately inhibiting ISGylation in cotransfection/ISGylation assays. However, whether UL50-p26 plays a regulatory role in viral infection needs further investigation.
Since Herc5, an E3 ligase for ISGylation, is associated with polyribosomes (58), abundantly expressed viral proteins may often be subjected to ISGylation in virus-infected cells. We previously showed that many viral proteins are ISGylated in cotransfection/ISGylation assays (12). The finding that pUL50 interacts with UBE1L and ISG15 prompted us to test whether pUL50 is a substrate for ISGylation. In cotransfection/ISGylation assays, pUL50 was indeed ISGylated within the central CR2 region of the N-terminal globular domain. Analysis of lysine-to-arginine mutants suggested that ISGylation occurred in several lysine residues in the CR2 region, making it difficult to further investigate the role of pUL50 ISGylation. It is conceivable that ISGylation of pUL50 may affect its targeting to the INM or interaction with pUL53. Determining whether ISGylation of pUL50 occurs during infection and affects its functions will require further study.
ISGylation appears to modulate both early and late events of HCMV infection. In our previous study, ISGylation enhancement by overexpression of E1, E2, and E3 enzymes and ISG15 reduced the activation of viral immediate early, early, and late promoters and the release of virions, while ISGylation reduction by short hairpin RNA (shRNA)-mediated Herc5 knockdown increased both viral gene expression and virion release (12). ISGylation of endosomal proteins was recently shown to promote the fusion of multivesicular bodies with lysosomes or autophagosomes and to inhibit exosome secretion and virus spread (59, 60). Considering that pUL50, which is expressed at a delayed early time after infection, targets the ER, is associated with ER-resident E3 ligase, and causes UBE1L degradation, it is tempting to study a regulatory role for pUL50 in these vesicle-related events.
MATERIALS AND METHODS
Cell culture, transfection, and reagents.
Human foreskin fibroblasts (HF cells), human embryonic kidney (HEK) 293T and 293A cells, and HeLa cells were grown in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum, penicillin (100 U/ml), and streptomycin (100 μg/ml). Nonessential amino acids (NEAA) were also added to the medium for HEK293A cells. DNA transfection of 293T, 293A, and HeLa cells was performed using the polyethylenimine (PEI) version of the cationic polymer procedure (61). Electroporation of HF cells was conducted using a Microporator MP-100 instrument (Digital Bio) as described previously (33). Interferon beta (IFN-β) was purchased from Chemicon. Cycloheximide (CHX) and the proteasome inhibitor MG132 were purchased from Sigma.
Viruses.
Recombinant HCMV (Toledo strain) was produced by transfecting a Toledo bacmid gifted from Hua Zhu (UMDNJ-New Jersey Medical School) into HF cells (62). The IE1-deleted CR208 virus (Towne strain) (63), which was provided by Edward Mocarski (Emory University School of Medicine), was grown in IE1-expressing HF cells (34). HCMV (AD169 strain)-GFP UL50-HA (52) was grown in HF cells. UV-inactivated viruses were produced by irradiating the virus stock with UV light three times at 0.72 J/cm2, using a model CL-1000 cross-linker (UVP).
Expression plasmids.
Mammalian expression plasmids for Myc-UBE1L (pMK86), Myc-UbcH8 (pMK92), Myc-Herc5 (pMK89), and SRT-UL50 (pMK55) were produced in the pcDNA6 vector (Life Technologies) by using Gateway technology. A plasmid (pCS3-MT) expressing Myc-ISG15GG (an active form of ISG15) (pYJ12) and plasmids (pSG5) expressing Flag-UbcH8 (pYJ23) and HA-Herc5 (pYJ29) were previously described (12). pcDNA3.1-UL50-HA was previously described (42). The following mutant versions of pcDNA3.1-UL50-HA were produced using Stratagene QuikChange site-directed mutagenesis: the 1-358 (pMK132), Δ359-381 (ΔTM) (pMK183), and M199V (pMK113) mutants. RNF170 variant 1 (RNF170v1) and variant 3 (RNR170v3) were PCR amplified from the cDNA and from a plasmid expressing GFP-RNF170 (a gift from Ho Chul Kang [Ajou University]), respectively, and subcloned into a pcDNA3.1 derivative containing 3×Flag, which was provided by Cheol-Sang Hwang (Pohang University of Science and Technology), to create plasmids expressing C-terminally Flag-tagged RNF170 proteins. The plasmid expressing HA-ubiquitin (HA-Ub) was previously described (62).
Adenoviral and retroviral vectors.
Adenoviral vectors expressing pUL50-HA (pMK181) were produced in pAD/CMV/V5-DEST (Invitrogen) by using Gateway technology. Recombinant adenoviruses were grown in 293A cells after transfection with adenoviral vector DNAs that were linearized by use of the PacI restriction enzyme. Retroviral vectors expressing pUL50-HA (pMK85) were produced on the background of pMIN (64) by using Gateway technology. To grow recombinant retroviruses, 293T cells were transfected with retroviral vectors and packaging plasmids pHIT60 (expressing the Gag and Pol proteins of murine leukemia virus) and pMD-G (expressing the envelope G protein of vesicular stomatitis virus). At 48 h, the culture supernatants were collected and used to transduce HF cells in the presence of Polybrene (7.5 μg/ml). The transduced cells were selected with G418 (0.4 mg/ml) and maintained in medium containing G418 (0.1 mg/ml).
Reverse transcription-PCR (RT-PCR).
Total RNAs were isolated from virus-infected cells by use of Tri reagent (Molecular Research Center, Inc.) and a MaXtract High Density tube (Qiagen). cDNAs were synthesized using the random hexamer/oligo(dT) primers in a QuantiTect reverse transcriptase kit (Qiagen). PCR was performed using the following primers: UBE1L forward, 5′-AGGTGGCCAAGAACTTGGTT-3′; UBE1L reverse, 5′-CACCACCTGGAAGTCCAACA-3′; β-actin forward, 5′-AGCGGGAAATCGTGCGTG-3′; and β-actin reverse, 5′-CAGGGTACATGGTGGTGCC-3′. The relative DNA levels in agarose gel images were quantitated using ImageJ (NIH).
Antibodies.
Anti-HA rat monoclonal antibody (MAb) 3F10 and anti-Myc mouse MAb 9E10, which were conjugated to horseradish peroxidase (HRP) or labeled with fluorescein isothiocyanate (FITC), were purchased from Roche. Mouse MAb 810R, detecting IE1 and IE2, was purchased from Chemicon. Anti-β-actin and anti-Flag mouse MAbs were purchased from Sigma. A rabbit polyclonal antibody (PAb) to UBE1L was obtained from Abcam. A mouse MAb against the SRT epitope has been described previously (65). An HRP-conjugated anti-green fluorescent protein (anti-GFP) mouse MAb was purchased from Santa Cruz. A rabbit anti-peptide PAb for pUL50 was raised against a mixture of the synthetic peptides N′-C-RTAGKRSSRTAS-C′ (pUL50 residues 205 to 216), N′-C-DVGGSARRPLEE-C′ (residues 297 to 318), and N′-C-RARSGPSRPQSG-C′ (residues 346 to 357).
Immunoblot analysis.
Cells were washed with phosphate-buffered saline (PBS), and total cell lysates were prepared by boiling the cell pellets in sodium dodecyl sulfate (SDS) loading buffer. Equal amounts of the clarified cell extracts were separated in an SDS-polyacrylamide gel and electroblotted onto nitrocellulose membranes. The blots were blocked with PBS plus 0.1% Tween 20 (PBST) containing 5% nonfat dry milk by incubation for 1 h at room temperature. After being washed with PBST three times, the blots were incubated with the appropriate antibodies in PBST for 1 h at room temperature. Antibody dilutions were 1:2,500 for anti-Flag, 1:10,000 for anti-SRT, 1:1,000 for anti-UL50, 1:10,000 for anti-UBE1L, and 1:1,000 for anti-ISG15 antibodies. After three 5-min washes with PBST, the blots were incubated with HRP-conjugated goat anti-mouse IgG or anti-rabbit IgG (Amersham) at a 1:5,000 dilution for 1 h at room temperature. HRP-conjugated anti-HA, anti-Myc, and anti-GFP antibodies were used at a 1:5,000 dilution. The blots were then washed three times with PBST, and the protein bands were visualized by use of an enhanced chemiluminescence system (Amersham). The relative protein levels in immunoblots were quantitated using ImageJ (NIH).
Indirect immunofluorescence assay (IFA).
Cells in chamber slides were fixed in 4% paraformaldehyde for 5 min and permeabilized in PBS containing 0.2% Triton X-100 for 20 min at 4°C. The cells were then incubated with appropriate primary antibodies (anti-HA at a 1:200 dilution; anti-Myc and anti-Flag at a 1:2,000 dilution) in PBS for 1 h at 37°C, followed by incubation with appropriate secondary antibodies at a 1:100 dilution for 1 h at 37°C. FITC-conjugated anti-HA antibody was also used at a 1:100 dilution. Mounting solution containing Hoechst dye and an antifade reagent (Molecular Probes) was used. For double labeling, two different antibodies were incubated together. Slides were examined and photographed by use of a Carl Zeiss LSM710Meta confocal microscope system.
Co-IP assays.
Cell lysates were prepared by sonication in 1 ml co-IP buffer (50 mM Tris-Cl [pH 7.4], 50 mM NaF, 5 mM sodium phosphate, 150 mM NaCl, 0.1% NP-40, protease inhibitors [Sigma]) by use of a Vibra-Cell microtip probe (Sonics and Materials) for 10 s (pulse on, l s; pulse off, 3 s). Cell lysates were incubated with appropriate antibodies (10 μg) for 16 h at 4°C. Next, 30 μl of a 50% slurry of protein A- and G-Sepharose (Amersham) was added, and the mixture was incubated for 2 h at 4°C to allow adsorption. The mixture was then pelleted and washed seven times with co-IP buffer. The beads were resuspended and boiled for 5 min in loading buffer. Each sample was analyzed by SDS-PAGE and immunoblotting with appropriate antibodies.
UBE1L ubiquitination assays.
293T cells were cotransfected with plasmids expressing target (Myc-UBE1L) or effector proteins and a plasmid expressing HA-Ub, and ubiquitination assays were performed as described previously (62). In brief, 24 h after transfection, cells were harvested and cell pellets were resuspended with 2% SDS lysis buffer containing protease inhibitors (Sigma) and boiled for 10 min. Cell lysates were diluted 10-fold with co-IP buffer and sonicated using a Vibra-Cell microtip probe. The clarified cell lysates were immunoprecipitated with anti-Myc antibody and then immunoblotted with anti-HA antibody as described above.
ACKNOWLEDGMENTS
We thank Dong-Er Zhang, Chin Ha Chung, Ho Chul Kang, and Cheol-Sang Hwang for providing plasmids and antibodies. We also thank Edward Mocarski and Hua Zhu for mutant and recombinant viruses.
This work was supported by grants from the Korean Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea (grant HI14C2114); the National Research Foundation of Korea (NRF), funded by the Ministry of Education (grant 2016R1A2B4011848); and the Deutsche Forschungsgemeinschaft (grant DFG MA1289/11-1).
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