FIG 4 .

Measuring EBV genome amplification and replication in HK1-EBV ALI culture. (A) Quantitative PCR measuring EBV genome equivalents in HK1 ALI-cultured EBV-infected cells. The averaged value was calculated from three biological triplicates, and each biological triplicate value and each error were determined from three technical replicates. Encapsidated genomes are DNase resistant (+DNase), and total (encapsidated and nonencapsidated) genomes were determined in the absence of DNase treatment (-DNase). (B) Termini assay of ALI-cultured HK1-EBV cells in comparison to zebra (Z)- and gB-transfected monolayer-cultured cells. Shown is a Southern blot of BamHI-digested genomic DNA hybridized to a random-primed and radiolabeled Xho1.9 fragment DNA probe located on the BamHI-digested fragment leftward of the EBV terminal repeats. Data are displayed from the same blot and exposure time. Intervening lanes were cropped for labeling purposes and are demarcated by a vertical dotted line.