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. 2018 Jul 20;15:50. doi: 10.1186/s12977-018-0431-4

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Minimal CT region of 4-2.J41wt Env required for restoring native antigenicity/conformation. a Schematic representation of CHD deleted mutants of 4-2.J41 Env proteins. b FACS-based cell surface staining assay of CHD deleted mutants with the bNAb, VRCO1 and non-NAb, F105. c Binding of CHD deleted mutants to CD4bs-directed non-NAb, b6 and CD4i-directed non-NAb, 17b at 20 µg/ml concentration. The error bars indicate the standard mean obtained from two independent experiments run in duplicates (P < 0.0001)