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. 2018 Jul 20;15:50. doi: 10.1186/s12977-018-0431-4

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — CT-deleted JRCSF but not JRFL and A5, have altered cell surface antigenicity/conformation. a, c, e FACS based cell surface staining assays of JRCSF, JRCSFΔCT with bNAbs, PGT126, PG9 and non-NAbs, F105, 412d; JRFL, JRFLΔCT with bNAbs, b12, PGT126 and non-NAbs, b6 and 412d; A5, A5ΔCT with bNAbs, PGT128 and PG9 and non-NAbs, F105, 39F over a range of antibody concentrations. b, d, f FACS based cell surface staining assays of JRFLE168K, JRFLE168KΔCT, A5, A5ΔCT, JRCSF, JRCSFΔCT with trimer-selective, cleavage-specific bNAb, PGT145 and non-NAbs, 17b (CD4i) and 19b (V3) over a range of antibody concentrations