A. Whole gut transit of Evans blue dye. After gavage of dye, the time for expulsion of the first blue pellet was longer in tgr5-ko mice than tgr5-wt or tgr5-tg mice, indicating delayed transit. B. Expulsion of a glass bead from the colon. After insertion of a glass bead into the colon, the time for expulsion was less in tgr5-tg mice than in tgr5-wt or tgr5-ko mice, indicating accelerated colonic transit. **P< 0.01, ***P<0.001 to tgr5-wt.