Table 2.

Cytotoxic effect of investigated lactones against L929 cells.^[a]

	Concentration [μg/mL]					Controls
	50	20	10	5	0.5	C+	C‐	DMSO
	Viable cells [%]								IC50  [μg/mL]
5 a	7*±0.010	16*±0.018	26*±0.011	32*±0.015	39*±0.018				0.39
5 b	16*±0.015	34*±0.014	54*±0.011	60*±0.010	81±0.018				9.26
5 c	15*±0.02	29*±0.019	32*±0.010	45*±0.010	54*±0.017				0.46
6 a	45*±0.011	55*±0.016	74±0.015	98±0.010	99±0.014				18
6 b	34*±0.011	39*±0.010	45*±0.018	53*±0.012	66±0.015	100±0.015	0	79±0.016	4.7
6 c	14*±0.012	28*±0.011	50*±0.019	67*±0.017	70±0.012				10
7 a	14*±0.013	34*±0.017	38*±0.014	45*±0.019	51*±0.012				0.49
7 b	26*±0.011	34*±0.016	45*±0.017	53*±0.019	63*±0.014				4.7
7 c	30*±0.015	36*±0.013	54*±0.015	66*±0.019	75±0.015				9.26

[a] The cytotoxicity was assessed by the MTT reduction assay. The cell viability was calculated for four experiments, including three repeats for each compound. Complete RMPI medium (cRPMI) was used as a positive control (C+) of cell viability (100 % viable cells) and 0.03 % H₂O₂ as a negative control (C−) of cell viability (100 % dead inactive cells). All values were expressed as the mean ± SD. The differences positive control and tested compounds were tested the non‐parametric Mann‐Whitney U test. Statistical significance: *p<0.05.