Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Saccharomyces cerevisiae) |
RAD52 | NA | SGD: YML032C | |
| Gene (S. cerevisiae) |
RAD51 | NA | SGD: YER095W | |
| Gene (S. cerevisiae) |
Srs2 | NA | SGD: YJL092W | |
| Genetic reagent (Bacteriophage φ-X174) |
viral (+) strand of φX174 DNA | |||
| Strain, strain background (S. cerevisiae) |
FF18733 | Cell line maintained in E. Coïc's lab |
||
| Strain, strain background (S. cerevisiae) |
YFP17; JKM146 | Cell line maintained in J. Haber's lab |
||
| Antibody | anti-Rad51 (rabbit polyclonal) | Abcam | ab63798 | |
| Antibody | anti-FLAG (Mouse polyclonal) | Sigma | F3165 | |
| Antibody | anti-DPS1 (Rabbit polyclonal) | S. Marcand's lab | ||
| Antibody | anti-RPA (Rabbit polyclonal) | V. Géli's lab | ||
| Antibody | anti-Mouse IR800 Goat monoclonal) | Advansta | R-05061–250 | |
| Antibody | anti-Rabbit IR700 Goat monoclonal) | Advansta | R-05054–250 | |
| Antibody | anti-Mouse IR700 Goat monoclonal) | Advansta | R-05055–250 | |
| Antibody | anti-Srs2 (Goat polyclonal) | Santa-Cruz | sc-11991 | |
| Recombinant DNA reagent | YCplac111-Rad52-6His-3FLAG | This study | progenitors: YCplac111 (GenBank: X75457, L26350) |
|
| Recombinant DNA reagent | Yiplac211-rad52-P381S; Yiplac211-rad52-Y376A |
This study | progenitors: Yiplac211 (GenBank: X75462, L26358) |
|
| Recombinant DNA reagent | pET15b-Rad52-FLAG | Progenitor: pET15b (addgene: 69661-3) | ||
| Peptide, recombinant protein |
Rad52-FLAG | This study | ||
| Peptide, recombinant protein |
RPA | This study | ||
| Peptide, recombinant protein |
Rad51 | This study | ||
| Peptide, recombinant protein |
Srs2 | This study | ||
| Peptide, recombinant protein |
3XFLAG | Sigma | F4799 | |
| Commercial assay or kit | GeneMorph II EZClone Domain Mutagenesis |
Agilent Technologies | 200552–5 | |
| Commercial assay or kit | Dynabeads coupled to Protein A | Invitrogen | 11041 | |
| Commercial assay or kit | Dynabeads coupled to PanMouse | Invitrogen | 10002D | |
| Commercial assay or kit | Platinum SYBR Green qPCR SuperMix-UDG |
Invitrogen | 11733–046 | |
| Software, algorithm | PSI-Blast | Altschul et al. (1997) | ||
| Software, algorithm | Muscle | Edgar (2004) | ||
| Software, algorithm | Jalview | Waterhouse et al. (2009) | ||
| Software, algorithm | iTEM software | Olympus, Soft Imaging Solutions | ||
| Software, algorithm | ImageQuant TL software | GE Healthcare Life Sciences |