Fig. 1.

The visible-light OCM and the tissue processing pipeline. (a) The visible light OCM system (Col. = Collimator). (b) The extracted brain tissue is embedded in 5% agarose. A vibratome is used to section the brain-agarose block in $200\text{-}\mu \mathrm{m}$ slices. These sections are glued onto glass slides and are then optically cleared for 15 min following the SWITCH protocol.²⁴ The cleared sections are imaged with the OCM setup. An automated $x-y-z$ stage is integrated in the sample arm to acquire images with a large field-of-view.