Figure 3. Summary data for dendritic cells and macrophages populations in VAT from DbHET, db/db, DbHET ^Flt3l−/− and db^Flt3l−/db^Flt3l− mice (age 6-10 weeks).

Panel A: Increased numbers of CD11c⁺F4/80⁻ dendritic cells were detected in VAT of db/db mice compared to DbHET controls. Depletion of Flt3l gene significantly decreased CD11c⁺F4/80⁻ dendritic cell numbers in db^Flt3l−/db^Flt3l− mice. Panel B: Similarly, CD83⁺CD86⁺ dendritic cells populations were increased in db/db mice while Flt3l depletion reduced CD83⁺CD86⁺ dendritic cells numbers in db^Flt3l−/db^Flt3l− mice. Panel C: An increase of CD11c⁺F4/80⁺ macrophages was observed in VAT of db/db mice compared to DbHET controls. However, depletion of Flt3l gene did not affect macrophage numbers in db^Flt3l−/db^Flt3l− mice. Data are shown as mean ± SEM. *: P < 0.05. between db/db and DbHET mice. †: P < 0.05. between db/db and DbHET ^Flt3l−/−mice, ‡: P < 0.05. between db/db and db^Flt3l−/db^Flt3l− mice.