Skip to main content
. Author manuscript; available in PMC: 2019 Jun 19.
Published in final edited form as: Immunity. 2018 Jun 19;48(6):1119–1134.e7. doi: 10.1016/j.immuni.2018.04.024

Figure 1. Satb1 and Runx1 are PU.1 interacting molecules in early T cells.

Figure 1

(A), Total extracts from Myc-Flag-PU.1-expressing Scid.adh.2c2 cells were subjected to two- step affinity purification followed by SDS-PAGE and silver staining. Mass spectrometry identified several specific polypeptides for both Satb1 and Runx1.

(B), Top five gene ontology (GO) terms for PU.1 interacting molecules.

(C), Total extracts from Scid.adh.2c2 cells transduced with Myc-Flag-PU.1 WT or ETS were subjected to immune precipitation (IP) with anti-Flag mAb followed by immunoblotting (IB) with anti-Runx1, anti-Satb1 or anti-Myc Abs (left). IB from total nuclear lysates are also shown (right). Schematic representation of the Myc-Flag-tagged PU.1 WT and ETS constructs are shown (bottom). Two independent experiments were performed with similar results (A, C)(cf. Fig. S1, Table S1).