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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Biomaterials. 2018 Jun 26;179:29–45. doi: 10.1016/j.biomaterials.2018.06.032

Figure 12.

Figure 12

(A–B) Representative immunohistochemical images of bone near the (A) femoral condyle and (B) tibial plateau defects at 10x (scale bar = 200 μm), 20x (scale bar = 100 μm) and 40x (scale bar = 50 μm) magnification show differential sclerostin expression (yellow arrows) in the host bone. (C) Representative area of interest in the host bone at 4x magnification (scale bar = 500 μm). The voids between trabeculae and the bony surfaces enclosed by the dashed yellow lines were excluded from the analysis to minimize artefacts. (D) Quantification of sclerostin staining of sheep femoral plug and tibial plateau defect samples reveals a significant decrease in sclerostin expression in weight-bearing tibial plateau defects. *** p<0.0005.