Figure 5b:

Creation of rat model with orthotopic lung cancer. (a, b) Under x-ray imaging guidance, a 26-gauge needle was positioned in the lower lobe of the right lung for cell inoculation. Chest radiographs obtained in posteroanterior and lateral projections helped confirm the deposit of injected cell pellet mixed with x-ray contrast agent (arrowheads) in the lung. (c) Bioluminescence imaging 3 weeks after cell injection depicted bioluminescence signals emitted from the lung cancer (orange-yellow). (d) Gross pathologic and histologic assessments further helped confirm formation of adenocarcinoma within the right lower lobe (original magnification, ×200).