Cap2 evolutionary conserved domains are essential for growth under iron-deprived conditions. (A) Schematic diagram of wild-type (WT) and mutant derivatives of Cap2. aa, amino acids; CTD, C-terminal domain. (B) Growth phenotype of the wild-type strain, and strains bearing the various N-terminal and C-terminal mutations in Cap2. The strains were precultured in SC-LIM liquid medium, serially diluted and spotted on SC-LIM alone or on SC-LIM plus BPS, SC-LIM plus BPS and FAS, or SC-LIM plus FAS.