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. 2018 Aug 1;3(4):e00370-18. doi: 10.1128/mSphere.00370-18

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Copyright © 2018 Srivastav et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 2 — The C-terminal deletion mutant protein is expressed in iron-depleted medium. Whole-cell extracts from cap2Δ/Δ (lane 1), wild type (lanes 2 and 3), cap2-1 (lane 4), ΔC1 (lane 5), ΔC2 (lane 6), ΔC3 (lane 7), ΔC4 (lane 8), and cap2-2 (lane 9) strains were separated on 8 to 16% gradient SDS-polyacrylamide gels, transferred to nitrocellulose membranes, and probed with affinity-purified anti-Cap2 antibody. A 72-kDa nonspecific band that consistently appeared in all lanes as reported previously (9) is indicated by an asterisk.