Skip to main content
. 2018 Jul 6;10(7):359. doi: 10.3390/v10070359

Figure 2.

Figure 2

FUS represses KSHV reactivation in iSLK.219 cells. (A) iSLK.219 cells were treated with siRNA against FUS for 48 h, followed by doxycycline-induced reactivation of KSHV for 48 h. Knockdown efficiency in latency and 48 h post-reactivation was determined by western blot and is indicated beneath each lane. GAPDH was used as a loading control. (B) Fluorescent microscopy, at 10× magnification, of iSLK.219 cells depleted of FUS by siRNA in (A). (C) RT-qPCR of viral transcripts from FUS-depleted iSLK.219 cells in (A). All samples were normalized to 18S and siCon levels set to 1. (D) Western blot of viral proteins from cells in (A). GAPDH was used as a loading control. Student t test used to determine statistical significance * p ≤ 0.05. ** p ≤ 0.005.