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. 2018 Aug 3;5(4):ENEURO.0067-18.2018. doi: 10.1523/ENEURO.0067-18.2018

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Copyright © 2018 Chatila et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 3. — Loss of BACE1 alters neuronal fate differentiation of NPCs. A, Representative confocal images of BrdU⁺ cells colabeled with NeuN (upper panels) or GFAP (lower panels). Arrows indicate the position of BrdU⁺ cells. Scale bar: 20 µm. B, Percentage of BrdU⁺ cells colabeled with NeuN or GFAP, or neither of them (mean ± SEM; n = 6 in BACE1^+/+, 8 in BACE1^+/–, and 5 in BACE1^–/– mice). For NeuN, F_(2,16) = 9.501, p = 0.0019. For GFAP, F_(2,16) = 0.786, p = 0.4725. For neither, F_(2,16) = 7.551, p = 0.0049; *, p < 0.05; **, p < 0.01. C, Representative confocal images of BrdU⁺ cells colabeled with DCX but not NeuN (upper panels), or with DCX and NeuN (lower panels). D, Percentage of BrdU⁺ cells colabeled with NeuN or DCX, both, or neither of them (mean ± SEM; n = 6 in BACE1^+/+, 8 in BACE1^+/–, and 5 in BACE1^–/– mice). For NeuN, F_(2,16) = 28.34, p < 0.0001. For DCX, F_(2,16) = 1.566, p = 0.2392. For both, F_(2,16) = 0.03229, p = 0.9863. For neither, F_(2,16) = 13.63, p = 0.0004; **, p < 0.01. E, Representative confocal images of BrdU⁺ cells colabeled with O2. F, Percentage of BrdU⁺ cells colabeled with NeuN or O2, or neither of them (mean ± SEM; n = 4 in BACE1^+/+, 6 in BACE1^+/–, and 4 in BACE1^–/– mice). For NeuN, F_(2,11) = 5.607, p = 0.0210. For O2, F_(2,11) = 1.235, p = 0.3283. For neither, F_(2,11) = 7.3, p = 0.0096; *, p < 0.05. G, Representative confocal images of GFAP⁺ astrocytes in the DG of BACE1^+/+, BACE1^+/–, and BACE1^–/– mice. Red, GFAP⁺ cells; blue, NeuN⁺ cells. Scale bar: 50 µm. H, Quantification of GFAP⁺ cells (mean ± SEM; n = 5 per group; F_(2,12) = 0.5162, p = 0.6095). I, GFAP signal intensity (mean ± SEM; n = 5 per group; F_(2,12) = 0.0344, p = 0.9662).