Table 6.
Summary of studies comparing cfDNA isolation methods. *pooled plasma samples. FL, fluorometry.
| Author (et al) | Subjects (n) | Isolation methods | Quantification method |
|---|---|---|---|
| Board | Healthy (5) | QIAamp® Mini Blood Kit | qPCR (AAT, 77 bp) |
| GENFIND™ Blood & Serum Genomic DNA Isolation Kit | |||
| QIAamp® Virus Spin Kit | |||
| ChargeSwitch® gDNA Serum Kit | |||
| Devonshire | Healthy (5)* | QIAamp® Circulating Nucleic Acid Kit | qPCR (TERT, 79 bp; ALUJ, various length) |
| NucleoSpin® Plasma XS Kit | |||
| FitAmp® Plasma/Serum DNA Isolation Kit | |||
| QIAamp® DNA Blood Mini Kit | |||
| Fleischhacker | Cancer (36), benign disease (8) | QIAamp® DNA Blood Mini Kit | qPCR (ERV, 135 bp; GAPDH, 92 bp; HBB, 101 bp) |
| NucleoSpin® Plasma F Kit | |||
| MagaPure® LC DNA Isolation Kit | |||
| Fong | Healthy (5)* | QIAamp® DNA Blood Midi Kit | FL, qPCR (CHD1, 68 bp, ACTB, 115 bp) |
| Puregene DNA Purification System Cell and Tissue Kit | |||
| ChargeSwitch® gDNA Serum Kit | |||
| ZR Serum DNA Kit™ | |||
| Kloten | Healthy (8) Cancer (50) |
Maxwell RSC | qPCR (KRAS) |
| ccfDNA Plasma kKt and QIAamp | |||
| Circulating Free Nucleic Acid Kit | |||
| Mauger | Cancer (4), healthy (1) | QIAamp® Circulating Nucleic Acid Kit | qPCR (KPN, 55 bp) |
| Norgen Plasma/Serum Cell-Free Circulating DNA | |||
| Norgen Plasma/Serum Circulating DNA Purification Mini Kit | |||
| NucleoSpin® Plasma XS Kit | |||
| Chemagic NA Extraction Kit | |||
| Mazurek | Cancer (10) | QIAamp® DNA Blood Mini Kit | qPCR (TERT, 98 bp) |
| QIAamp® Circulating Nucleic Acid Kit | |||
| Sherlock AX kit | |||
| Page | Healthy (1) | QIAamp® DNA Blood Mini Kit QIAamp® Circulating Nucleic Acid Kit NucleoSpin® Plasma XS Kit FitAmp® Plasma/Serum DNA Isolation Kit |
qPCR (GAPDH, 96 bp, ACTBL2 67 bp and HPRT1 64 bp) |
| Sorber | Cancer (10) | QIAamp® Circulating Nucleic Acid Kit | FL |
| PME free-circulating DNA Extraction Kit | |||
| Maxwell® RSC ccfDNA Plasma Kit | |||
| EpiQuikTM Circulating Cell-Free DNA Isolation Kit | |||
| NEXTprep-MagTM cfDNA Isolation Kit v1 | |||
| NEXTprep-MagTM cfDNA Isolation Kit v2 | |||
| van Ginkel | Healthy (4) | QIAamp® Circulating Nucleic Acid Kit | dPCR (BRAF, 91 bp) |
| PME free-circulating DNA Extraction Kit | |||
| Quick-cfDNA™ Serum & Plasma Kit | |||