Table 3.
Protein expression in mammary gland
| Protein | LIACa (n = 12) | HIAC (n = 12) | P-value |
|---|---|---|---|
| p-Akt (Ser473) | 1.31 ± 0.22 | 0.41 ± 0.07 | 0.001 |
| Akt | 2.22 ± 0.26 | 1.23 ± 0.16 | 0.004 |
| p-Akt (Ser473)/Akt Ratio | 0.65 ± 0.11 | 0.36 ± 0.05 | 0.025 |
| p-AMPK (Thr172) | 5.08 ± 0.41 | 6.55 ± 0.53 | 0.040 |
| AMPK | 1.30 ± 0.12 | 1.01 ± 0.06 | 0.043 |
| p-AMPK (Thr172)/AMPK Ratio | 4.04 ± 0.54 | 6.44 ± 0.47 | 0.003 |
| p-PRAS40 (Thr246) | 0.51 ± 0.05 | 0.26 ± 0.03 | 0.001 |
| PRAS40 | 0.41 ± 0.03 | 0.28 ± 0.02 | 0.005 |
| p-PRAS40 (Thr246)/ PRAS40/ Ratio | 1.26 ± 0.07 | 0.92 ± 0.06 | 0.001 |
| p-4EBP1 (Thr37/46) | 2.06 ± 0.18 | 1.16 ± 0.14 | 0.001 |
| 4EBP1 | 1.42 ± 0.11 | 0.91 ± 0.11 | 0.003 |
| p-4EBP1 (Thr37/46)/4EBP1 Ratio | 1.47 ± 0.09 | 1.32 ± 0.07 | 0.204 |
| p-p70S6K (Thr389) | 0.44 ± 0.06 | 0.41 ± 0.04 | 0.676 |
| p70S6K | 0.42 ± 0.03 | 0.27 ± 0.03 | 0.004 |
| p-p70S6K (Thr389)/ p70S6K Ratio | 1.23 ± 0.23 | 1.70 ± 0.20 | 0.143 |
| p-IRS-1 (Ser636/639) | 0.03 ± 0.003 | 0.01 ± 0.003 | 0.005 |
| IRS-1 | 0.10 ± 0.02 | 0.10 ± 0.01 | 0.884 |
| p-IRS-1 (Ser636/639)/IRS-1 Ratio | 0.40 ± 0.12 | 0.14 ± 0.02 | 0.037 |
| p-PTEN (Ser380) | 1.37 ± 0.08 | 1.00 ± 0.10 | 0.011 |
| PTEN | 0.72 ± 0.05 | 0.51 ± 0.05 | 0.007 |
| p-PTEN (Ser380)/PTEN Ratio | 1.92 ± 0.04 | 1.97 ± 0.05 | 0.387 |
| BAX | 2.03 ± 0.15 | 2.51 ± 0.12 | 0.021 |
| BCL2 | 0.93 ± 0.21 | 1.46 ± 0.36 | 0.222 |
IRS-1, insulin receptor substrate-1.
aValues are peak heights normalized to a loading control, mean ± SEM.