Table 2.
Gene target | Primer sequence (5′ to 3′) |
---|---|
rIns1 | F – CCTGCTCGTCCTCTGGGAGCCCAAG R – CTCCAGTGCCAAGGTCTGAAGATCC |
rIns2 | F – CCTGCTCATCCTCTGGGAGCCCCGC R – CTCCAGTGCCAAGGTCTGAAGGTCA |
rPIAS1 | F – CTACCAGCCTACGGGTTTCG R – GAACAGGTAAGTGCCCGACA |
rPIASy | F – ATAGATGGGCTGCTGTCGAAGA R – ATTGGGCGCCATGAACCTT |
rGlis3 | F – GTGAAGGCACATTCTTCCAAAGA R – GGAGATCTGGATGGAGCTCAGT |
FLAG-Glis3 | F – TGGACTACAAAGACCATGACGG R – GGGCTCTGATGGGAGGGATA |
rUcp2 | F – GCATTGGCCTCTACGACTCT R – CTGGAAGCGGACCTTTACC |
18s rRNA | F – GTAACCCGTTGAACCCCATT R – CCATCCAATCGGTAGTAGCG |