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. 2018 Jul 25;4(7):e00709. doi: 10.1016/j.heliyon.2018.e00709

Table 2.

List of primers used for RT-PCR.

Gene target Primer sequence (5′ to 3′)
rIns1 F – CCTGCTCGTCCTCTGGGAGCCCAAG
R – CTCCAGTGCCAAGGTCTGAAGATCC
rIns2 F – CCTGCTCATCCTCTGGGAGCCCCGC
R – CTCCAGTGCCAAGGTCTGAAGGTCA
rPIAS1 F – CTACCAGCCTACGGGTTTCG
R – GAACAGGTAAGTGCCCGACA
rPIASy F – ATAGATGGGCTGCTGTCGAAGA
R – ATTGGGCGCCATGAACCTT
rGlis3 F – GTGAAGGCACATTCTTCCAAAGA
R – GGAGATCTGGATGGAGCTCAGT
FLAG-Glis3 F – TGGACTACAAAGACCATGACGG
R – GGGCTCTGATGGGAGGGATA
rUcp2 F – GCATTGGCCTCTACGACTCT
R – CTGGAAGCGGACCTTTACC
18s rRNA F – GTAACCCGTTGAACCCCATT
R – CCATCCAATCGGTAGTAGCG