Table 3. . Mean methylation level of GRHL3 gene in neural tube defect cases and controls.
| Group | n | Mean methylation (%) | OR† (95% CI) | Adjusted-OR (95% CI) |
|---|---|---|---|---|
| NTDs | 46 | 16.5 (14.0,19.0) | 1.552 (1.232–1.954) | 1.657 (1.279–2.146) |
| Controls | 23 | 20.1 (16.9,23.3) | 1.000 | 1.000 |
| Spinal bifida | 26 | 15.9 (13.4,18.4) | 1.514 (1.121–2.045) | 1.562 (1.140–2.140) |
| Controls_1 | 13 | 19.3 (15.9,22.7) | 1.000 | 1.000 |
| Anencephaly | 20 | 17.4 (15.0,19.8) | 1.800 (1.165–2.780) | 2.114 (1.178–3.792) |
| Controls_2 | 10 | 21.1 (18.5,23.7) | 1.000 | 1.000 |
Using logistic regression to investigate the relationship between methylation of GRHL3 with NTDs and subtypes of NTDs.
†In order to make it better to explain the regression coefficient, we take the negative value of the methylation data into the model.
Adjusted-OR: Adjusted by folic acid supplementation; CI: Confidence interval; control_1: The control matched with spinal bifida case; control_2: The control matched with anencephaly case; NTD: Neural tube defect; OR: Odds ratio.