a, Cohesin accumulation in the 3’ region of Tspan5 and Rap1gds1. Binding of CTCF, Nipbl, Stag1 and Scc1 was determined by ChIP-seq, and the transcriptional activity was measured by GRO-seq in wild-type MEFs. b, c, Cohesin island formation in Ctcf Wapl DKO cells at convergent gene pairs with different transcriptional activity. Individual example (b) and Scc1 density plots (c) for each category is shown. d, Density profiles of Scc1 binding for equally transcribed gene pairs with different transcriptional activity (TPM > 5, 3-5 and 1-3). e, Presence of cohesin islands at the actively transcribed isolated Ext1 gene. f, Scc1-binding profile for all isolated transcribed genes (TPM 5-9).