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. Author manuscript; available in PMC: 2018 Aug 7.
Published in final edited form as: Nature. 2017 Apr 19;544(7651):503–507. doi: 10.1038/nature22063

Figure 4. Transcription is required for the generation of cohesin islands.

Figure 4

a, Binding of Scc1 and mRNA profiling in DKO cells upon serum stimulation at the Fam129b/Lrsam1 gene pair. b, Shift in cohesin island positioning is visualized by the cumulative sum of reads per nucleotide (see Online Methods). c, Loss of cohesin island at the Bzw1/Clk1 gene pair upon transcriptional inhibition by actinomycin D in DKO cells. d. Density profiles of Scc1 accumulation at convergently transcribed genes in response to actinomycin D treatment. e. Dis- and re-apperance of a cohesin island upon temporal inhibition of RNA polymerase II by DRB at the Bzw1/Clk1 gene pair in DKO cells. f. Density profiles of Scc1 accumulation at convergently transcribed genes in response to DRB treatment. g, Model of cohesin movement and accumulation at transcribed genes. Cohesin rings are indicated by red circles and cohesin peaks as determined by ChIP-seq by black triangles.