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Different culture vessels used to test hemocyte attachment
| Plating technique | |||
|---|---|---|---|
| Culture vessel | Surface area (cm2) | Optimal medium volume (mL) | Cell attachment |
| 35 mm petri dish (Terumo (Guyancourt, France) 601–5412) | 8 | 2 | – |
| 35 mm petri dish (Terumo 601–5412) + glass slip | 8 | 2 | + |
| Corning 12-well plate (734–2334) | 1.9 | 0.5 | – |
| Corning 96-well plate (734–1376) | 0.32 | 0.2 | + |
Cells were visually observed to identify if attachment occurred (+: attachment occurred; −: cells did not attach)
