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. 2018 Aug 9;7(8):57. doi: 10.1038/s41389-018-0071-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — Morphology of SGC7901-LGR5 cells was evaluated by phase-contrast microscopy. Overexpression of LGR5 promotes cell morphological changes, acquiring fibroblast-like appearance with spindle shape (a, b). Forty-eight hours after transfection, the number of spindle shape cell of SGC7901-LGR5 is significantly more than that of SGC7901-shRNA-LGR5 and control groups. The number of spindle shape and round shape cells in SGC7901-LGR5 is more than that of SGC7901-shRNA-LGR5 and control groups. The SGC7901-LGR5 and SGC7901-shRNA-LGR5 were treated with C59 and Wnt3 to restore the cellular phenotypes. c, d Rhodamine phalloidin staining of actin filament in gastric adenocarcinoma cells SGC7901 and BGC823. Cells were grown to semi-confluency on poly-l-lysine/laminin, coated, fixed, and stained with rhodamine phalloidin as described in Methods. The actin stress fibers (red) were stained throughout the cells. The cell nuclei were counterstained with DAPI (blue). LGR5 overexpression and knockdown cells were also treated with C59 and Wnt3 to restore the cellular phenotypes. Images of close-ups on pseudopods were in the lower panel. Scale bar = 10 μm

Fig. 7 — Morphology of SGC7901-LGR5 cells was evaluated by phase-contrast microscopy. Overexpression of LGR5 promotes cell morphological changes, acquiring fibroblast-like appearance with spindle shape (a, b). Forty-eight hours after transfection, the number of spindle shape cell of SGC7901-LGR5 is significantly more than that of SGC7901-shRNA-LGR5 and control groups. The number of spindle shape and round shape cells in SGC7901-LGR5 is more than that of SGC7901-shRNA-LGR5 and control groups. The SGC7901-LGR5 and SGC7901-shRNA-LGR5 were treated with C59 and Wnt3 to restore the cellular phenotypes. c, d Rhodamine phalloidin staining of actin filament in gastric adenocarcinoma cells SGC7901 and BGC823. Cells were grown to semi-confluency on poly-l-lysine/laminin, coated, fixed, and stained with rhodamine phalloidin as described in Methods. The actin stress fibers (red) were stained throughout the cells. The cell nuclei were counterstained with DAPI (blue). LGR5 overexpression and knockdown cells were also treated with C59 and Wnt3 to restore the cellular phenotypes. Images of close-ups on pseudopods were in the lower panel. Scale bar = 10 μm