Table 1.

Tools and databases

Tools/databases	Description
NGSengine	Platform‐independent software for the high‐resolution identification of HLA alleles by NGS (http://www.gendx.com/ngs-engine-download).
DR2S	Dual Redundant Reference Sequencing (DR2S) is an R package designed to facilitate the generation of reliable, full‐length phase‐defined reference sequences for novel HLA alleles (https://github.com/gschofl/DR2S).
TypeLoader	Automatic EMBL‐ENA and IPD‐IMGT/HLA Database Upload Generator. This web service takes FASTA and GenDx generated XML files, processes them and automatically generates data for direct submission to the EMBL‐ENA and the IPD‐IMGT/HLA Database (https://github.com/DKMS-LSL/typeloader).
ENA	The EMBL‐ENA provides a comprehensive record of the world's nucleotide sequencing information, covering raw sequencing data, sequence assembly information and functional annotation (http://www.ebi.ac.uk/ena).
IPD‐IMGT/HLA Database	The IPD‐IMGT/HLA Database provides a specialist database for sequences of the MHC and includes the official sequences named by the WHO Nomenclature Committee for factors of the HLA System. The IPD‐IMGT/HLA Database is part of the international ImMunoGeneTics project (IMGT) (http://www.ebi.ac.uk/ipd/imgt/hla/).

EMBL‐ENA, European Molecular Biology Laboratory – European Nucleotide Archive; HLA, human leukocyte antigen; NGS, next generation sequencing; WHO, World Health Organization.