Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jul 25;159(8):1580–1591. doi: 10.1097/j.pain.0000000000001246

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2018 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the International Association for the Study of Pain.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal.

PMC Copyright notice

Figure 7. — Contributions of adenosine A1 receptors (A1Rs) and prostatic acid phosphatase (PAP) neurons to neuropathic pain hypersensitivity in resiniferatoxin (RTX) neuropathy. (A–F) Double-labeling immunofluorescence staining was performed on dorsal root ganglion (DRG) sections with anti-PAP (A, C, D, and F in green) and anti-A1R (B, C, E, and F in red) antisera in the vehicle group (A–C) and day 7 after RTX neuropathy (RTX group; D–F). (C and F) The images show colocalization of A1R and PAP DRG neurons in the vehicle (C) and RTX (F) groups. (G) The diagram indicates the ratios of A1R(+)/PAP(+) (open bar, n = 6) and PAP(+)/A1R(+) (filled bar, n = 6) in the vehicle group shown in (A–F). (H–L) The mechanical threshold was assessed through von Frey monofilament tests using up-and-down algorithm. The mechanical threshold was highly linearly correlated with the densities of PAP(+) (H, n = 16), A1R(+) (I, n = 11), and A1R(+):PAP(+) neurons (J, n = 11). By contrast, the density of A1R(−)/PAP(+) neurons (K, n = 11) showed a weak linear correlation with the mechanical threshold, and no linear correlation between A1R(+)/PAP(−) neurons (L, n = 11) and the mechanical threshold was evident. Bar, 50 µm.