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. 2018 May 2;37(32):4385–4397. doi: 10.1038/s41388-018-0278-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Radiation-induced CD105 expression in prostate cancer cells supports radio-resistance. a Cell surface CD105 expression was measured in cell lines at 72 h following a dose range of irradiation (0, 2, 4, or 6 Gy). b The durability of cell surface CD105 expression in 22Rv1 was determined 0, 0.5, 4, 8, 24, 48, 72, 120, and 168 h following 4 Gy irradiation. CD105 cell surface expression fold change was normalized to levels expressed prior to irradiation. c The mRNA expression of CD105 ligands was measured at 0, 4, and 8 h post radiation by rtPCR. Expression was normalized to GAPDH and to the 0 h time point. d Western blot for phosphorylated Smad1/5 was measured in 22Rv1 cells in the presence or absence of serum starvation and treatment with 50 ng/ml BMP4 or TRC105. β-actin expression served as the loading control. Molecular weight (kDa) is indicated. e Annexin-V expression was measured in 22Rv1 cells by FACS analysis 5 days following 4 Gy irradiation and treatment of IgG or TRC105. f Clonogenic assay was measured 10 days following irradiation of 22Rv1 and C4-2B cells in a dose range of 0–6 Gy in the presence of IgG or TRC105. Data are reported as a mean ± S.D. of three independent experiments (*p < 0.05, **p < 0.01, ***p < 0.001 compared to control, unless otherwise indicated)