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. 2018 Aug 1;9(1):1138–1149. doi: 10.1080/21505594.2018.1467710

Figure 3.

Figure 3.

Effect of point mutations in the capsule synthesis locus on bactericidal activity mediated by anti-ACWY capsule antiserum and anti-LPS mAb. Typical results are shown for selected reference and mutant isolates. Reference isolate 2602: sensitive against both anti-ACWY capsule antiserum and anti-LPS mAb; isolate 1976 lacking capsule due to a non-synonymous SNP in the ctrE gene: lack of activity of anti-ACWY capsule antiserum; isolate 2008 expressing more polysaccharide than the reference isolate as a result of a non-synonymous mutation in the csaB gene: generally reduced bactericidal effect irrespective of the specificity of the antibodies; isolate 1666 expressing non-O-acetylated capsular polysaccharide: lack of activity of anti-ACWY capsule antiserum. Error bars represent standard error of the mean titer of triplicate measurements in three independent experiments. The bottom dotted line indicates the lowest serum dilution (1:10) and highest mAb concentration (100 µg/mL) tested.