There are errors in Table 1. The amplicon lengths for primers RID_FWD/RID_REV and Mac_FWD/Mac_REV do not include primer regions. Please see the corrected Table 1 here.
Table 1. Cytochrome b markers and their corresponding primers.
| Genetic marker for: | Primer name: | Primer sequence (5’–3’): | Annealing temperature (°C): | Amplicon length* (bp): | Reference: |
|---|---|---|---|---|---|
| Diceros bicornis (black rhino) | Rh_BR_FWD (forward) | AATCTGCCTAATCCTACAAATC | 60 | 222 | This study |
| Rh_BR_REV (reverse) | GGTTTCTAGGAAGGTGTAGG | ||||
| Ceratotherium simum (white rhino) | Rh_WR_FWD (forward) | CCACTCATTCATCGATCTGC | 60 | 266 | This study |
| Rh_WR_REV (reverse) | TAATAGATACCGCGTCCTAC | ||||
| Rhinoceros unicornis (Indian rhino) | Rh_IR_FWD (forward) | TCTCACCCACTAGTTAAAATCA | 60 | 310 | This study |
| Rh_IR_REV (reverse) | AGGAAGGTGTAAGATCCATAG | ||||
| All rhino species | RID_FWD (forward) | AACATCCGTAAATCYCACCCA | 55 | 274 | [6] |
| RID_REV (reverse) | GGCAGATRAARAATATGGATGCT | ||||
| All rhino species | Mac_FWD (forward | CAYTATACACCAGACACAACAAC | 55 | 226 | This study |
| Mac_REV (reverse) | TGAAYGCDGTGGCTATTAGRG |
*Amplicon lengths are inclusive of primer regions.
Reference
- 1.Ewart KM, Frankham GJ, McEwing R, The DT, Hogg CJ, Wade C, et al. (2018) A rapid multiplex PCR assay for presumptive species identification of rhinoceros horns and its implementation in Vietnam. PLoS ONE 13(6): e0198565 10.1371/journal.pone.0198565 [DOI] [PMC free article] [PubMed] [Google Scholar]