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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Virology. 2018 Mar 28;518:358–368. doi: 10.1016/j.virol.2018.03.011

Figure 5.

Figure 5.

The M133T mutant could rescue virion secretion of the G145R or N146Q mutant in cis through the S protein. Huh7 cells seeded in 6-well plates were cotransfected with 1.2 μg of the 1.5mer replication construct, 0.2 μg of the 0.7mer L+/M+/S- construct, and 0.6 μg of the 0.7mer L-/M-/S+ construct. The M133T mutation accompanied the G145R (or N146Q) mutation either on the L+/M+/S- construct, or the L-/M-/S+ construct. Cells receiving just the L+/M+/S- construct or the L-/M-/S+ construct served as negative controls of virion secretion. A: Western blot analysis of intracellular envelope proteins. B: Quantification of HBsAg released to culture supernatant. C - E: real-time PCR for intracellular replicative DNA (C), extracellular capsid DNA (D) and virion DNA (E). F: Virion secretion efficiency.